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首页> 外文期刊>Journal of mass spectrometry: JMS >Simultaneous detection of phosphatidylcholines and glycerolipids using matrix-enhanced surface-assisted laser desorption/ionization-mass spectrometry with sputter-deposited platinum film
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Simultaneous detection of phosphatidylcholines and glycerolipids using matrix-enhanced surface-assisted laser desorption/ionization-mass spectrometry with sputter-deposited platinum film

机译：基质增强的表面辅助激光解吸/电离质谱联用溅射沉积铂膜同时检测磷脂酰胆碱和甘油脂

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摘要

Matrix-assisted laser desorption/ionisation (MALDI) imaging mass spectrometry (IMS) allows for the simultaneous detection and imaging of several molecules in brain tissue. However, the detection of glycerolipids such as diacylglycerol (DAG) and triacylglycerol (TAG) in brain tissues is hindered in MALDI-IMS because of the ion suppression effect from excessive ion yields of phosphatidylcholine (PC). In this study, we describe an approach that employs a homogeneously deposited metal nanoparticle layer (or film) for the detection of glycerolipids in rat brain tissue sections using IMS. Surface-assisted laser desorption/ionisation IMS with sputter-deposited Pt film (Pt-SALDI-IMS) for lipid analysis was performed as a solvent-free and organic matrix-free method. Pt-SALDI produced a homogenous layer of nanoparticles over the surface of the rat brain tissue section. Highly selective detection of lipids was possible by MALDI-IMS and Pt-SALDI-IMS; MALDI-IMS detected the dominant ion peak of PC in the tissue section, and there were no ion peaks representing glycerolipids such as DAG and TAG. In contrast, Pt-SALDI-IMS allowed the detection of these glycerolipids, but not PC. Therefore, using a hybrid method combining MALDI and Pt-SALDI (i.e., matrix-enhanced [ME]-Pt-SALDI-IMS), we achieved the simultaneous detection of PC, PE and DAG in rat brain tissue sections, and the sensitivity for the detection of these molecules was better than that of MALDI-IMS or Pt-SALDI alone. The present simple ME-Pt-SALDI approach for the simultaneous detection of PC and DAG using two matrices (sputter-deposited Pt film and DHB matrix) would be useful in imaging analyses of biological tissue sections. Copyright (c) 2015 John Wiley & Sons, Ltd.
机译：基质辅助激光解吸/电离（MALDI）成像质谱（IMS）允许同时检测和成像脑组织中的几种分子。但是，由于磷脂酰胆碱（PC）的离子产量过高而产生的离子抑制作用，因此在MALDI-IMS中阻碍了脑组织中甘油二脂（DAG）和三酰甘油（TAG）等甘油的检测。在这项研究中，我们描述了一种方法，该方法采用均匀沉积的金属纳米颗粒层（或膜）来使用IMS检测大鼠脑组织切片中的甘油脂。使用无溶剂和无有机基质的方法，进行了具有用于脂类分析的溅射沉积Pt膜的表面辅助激光解吸/电离IMS（Pt-SALDI-IMS）。 Pt-SALDI在大鼠脑组织切片的表面上产生了均匀的纳米颗粒层。 MALDI-IMS和Pt-SALDI-IMS可以对脂质进行高度选择性的检测。 MALDI-IMS检测到了组织切片中PC的主要离子峰，没有代表DAG和TAG等甘油脂的离子峰。相反，Pt-SALDI-IMS允许检测这些甘油脂，但不允许检测PC。因此，使用结合了MALDI和Pt-SALDI的混合方法（即基质增强[ME] -Pt-SALDI-IMS），我们实现了大鼠脑组织切片中PC，PE和DAG的同时检测，并且对这些分子的检测优于单独的MALDI-IMS或Pt-SALDI。使用两个矩阵（溅射沉积的Pt膜和DHB矩阵）同时检测PC和DAG的当前简单的ME-Pt-SALDI方法在生物组织切片的成像分析中很有用。版权所有（c）2015 John Wiley＆Sons，Ltd.

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