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首页> 外文期刊>Journal of mass spectrometry: JMS >Versatility of tandem mass spectrometry for focused analysis of oxylipids
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Versatility of tandem mass spectrometry for focused analysis of oxylipids

机译：串联质谱的多功能性可用于脂质的重点分析

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Liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the multiple reaction monitoring (MRM) scan mode has been the primary MS method applied for the target identification of specific and minor oxylipids in complex matrices, such as eicosanoids and docosanoids, which are potent lipid mediators derived from polyunsaturated fatty acid oxygenation. However, the high specificity of MRM can limit the detection of species with m/z MRM transitions not covered by the method. In addition to MRM, tandem-quadrupole mass analyzers enable other experiments to be conducted, by fragmenting ions via collision-induced dissociation process (CID). This paper presents the potential of tandem mass spectrometry for the focused analysis of oxylipids. We have successfully developed an LC-MS/MS method for the identification of precursor ions of m/z 115, a diagnostic product ion of 5-hydroxy- and 5-epoxy-fatty acids. As a proof of concept, the developed method was used to discover several oxylipids oxidized at C5 derived from arachidonic acid (C20 : 4) oxygenation in a hypothalamus rat extract that were not identified using the target MRM methodology. The proposed focused MS/MS-based approach in a tandem mass analyzer has proven to be a powerful strategy to accelerate the identification of oxylipids with structural similarities and assist the field of lipidomic research. Copyright (C) 2015 John Wiley & Sons, Ltd.
机译：多反应监测（MRM）扫描模式下的液相色谱-串联质谱（LC-MS / MS）是用于复杂基质中特定和次要脂质的目标识别的主要MS方法，例如类花生酸和二十二烷酸。是衍生自多不饱和脂肪酸氧合的有效脂质介体。但是，MRM的高特异性可能会限制该方法未涵盖的具有m / z MRM跃迁的物种的检测。除了MRM，串联四极杆质量分析仪还可以通过碰撞诱导解离过程（CID）裂解离子，从而进行其他实验。本文介绍了串联质谱法对脂质进行重点分析的潜力。我们已经成功地开发了一种LC-MS / MS方法，用于鉴定m / z 115的前体离子，该离子是5-羟基和5-环氧脂肪酸的诊断产物离子。作为概念的证明，所开发的方法用于在下丘脑大鼠提取物中发现在花生四烯酸（C20：4）氧化作用下从花生四烯酸（C20：4）氧化的C5处氧化的几种脂质，但未使用目标MRM方法进行鉴定。事实证明，在串联质谱分析仪中提出的基于MS / MS的聚焦方法是一种有效的策略，可加快结构相似性脂质的鉴定，并协助脂质组学研究领域。版权所有（C）2015 John Wiley＆Sons，Ltd.

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