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首页> 外文期刊>Journal of mass spectrometry: JMS >Commercial formaldehyde standard for mass calibration in mass spectrometry
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Commercial formaldehyde standard for mass calibration in mass spectrometry

机译：用于质谱仪质量校准的商用甲醛标准品

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摘要

Common calibration standards for mass spectrometry can be a source of many problems including instrument contamination, ionization suppression and formation of unidentified ions during subsequent analysis. In this article, we present a new approach for the calibration of mass analyzers such as a quadrupole-time-of-flight mass spectrometry using a diluted solution of commercial formaldehyde. Formaldehyde is an inexpensive and commonly used solvent, and its intrinsic polymerization leads to the formation of polyoxymethylene (POM) oligomers, which are excellent multiple calibration standards for a low-mass spectral region (up to m/z 400) in the positive and negative mode of electrospray ionization. We explore the nature and origin of these polymeric species and attributed them to chemical reactions of formaldehyde and stabilizing agents in commercial formaldehyde solutions and during electrospray ionization. In contrast to other calibrants, POM oligomers do not contaminate the instrument and can easily be removed from the sample delivery system. Using tandem mass spectrometry, we elucidate the structures of the detected POM oligomers and report their reference masses, which are tightly spaced by 30 mass units. In our calibration method, mass errors of <5ppm can be obtained from m/z 20-400 using external calibration with a simple one-point zero-order correction of spectral data and without the need for operation of a dual spray or internal calibrants. Our approach will be particularly useful for those interested in the analysis of fragile ions with low m/z values and can function at instrumental conditions required for analysis of the most labile metabolites and environmental contaminants. Copyright (c) 2015 John Wiley & Sons, Ltd.
机译：质谱的通用校准标准可能是许多问题的根源，包括仪器污染，电离抑制和后续分析过程中形成的未识别离子。在本文中，我们提出了一种用于质量分析仪校准的新方法，例如使用商业甲醛稀释溶液进行四极杆飞行时间质谱分析。甲醛是一种廉价且常用的溶剂，其固有的聚合作用会导致形成聚甲醛（POM）低聚物，对于低质量光谱区（正向和负向）（高达m​​ / z 400），聚甲醛都是极好的多重校准标准电喷雾电离的模式。我们探索了这些聚合物种类的性质和起源，并将其归因于甲醛和稳定剂在商业甲醛溶液中以及在电喷雾电离过程中的化学反应。与其他校准物相比，POM低聚物不会污染仪器，可以轻松地从样品输送系统中移除。使用串联质谱，我们阐明了检测到的POM低聚物的结构，并报告了它们的参考质量，这些质量以30个质量单位紧密间隔。在我们的校准方法中，使用外部校准对光谱数据进行简单的单点零阶校正即可从m / z 20-400获得小于5ppm的质量误差，而无需使用双重喷雾或内部校准剂。我们的方法对于那些对m / z值低的易碎离子感兴趣的人特别有用，并且可以在分析最不稳定的代谢物和环境污染物所需的仪器条件下发挥作用。版权所有（c）2015 John Wiley＆Sons，Ltd.

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