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Evaluation of mass spectrometric data using principal component analysis for determination of the effects of organic lakes on protein binder identification

机译:使用主成分分析评估质谱数据以确定有机湖泊对蛋白质结合剂鉴定的影响

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摘要

Matrix-assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry is commonly used for the identification of proteinaceous binders and their mixtures in artworks. The determination of protein binders is based on a comparison between the m/z values of tryptic peptides in the unknown sample and a reference one (egg, casein, animal glues etc.), but this method has greater potential to study changes due to ageing and the influence of organic/inorganic components on protein identification. However, it is necessary to then carry out statistical evaluation on the obtained data. Before now, it has been complicated to routinely convert the mass spectrometric data into a statistical programme, to extract and match the appropriate peaks. Only several homemade' computer programmes without user-friendly interfaces are available for these purposes.
机译:基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF)通常用于鉴定艺术品中的蛋白质粘合剂及其混合物。蛋白质结合剂的确定基于未知样品中胰蛋白酶肽与参考样品(例如蛋黄素,酪蛋白,动物胶等)的m / z值之间的比较,但是这种方法具有更大的潜力研究衰老引起的变化以及有机/无机成分对蛋白质鉴定的影响。但是,必须对获得的数据进行统计评估。在此之前,将质谱数据常规转换为统计程序以提取和匹配适当的峰已经很复杂。这些目的只有几个没有用户友好界面的自制计算机程序可用。

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