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首页> 外文期刊>Journal of mass spectrometry: JMS >Polarization induced electrospray ionization mass spectrometry for the analysis of liquid, viscous and solid samples
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Polarization induced electrospray ionization mass spectrometry for the analysis of liquid, viscous and solid samples

机译：极化诱导电喷雾电离质谱法，用于分析液体，粘性和固体样品

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In this study, a polarization-induced electrospray ionization mass spectrometry (ESI-MS) was developed. A micro-sized sample droplet was deposited on a naturally available dielectric substrate such as a fruit or a stone, and then placed close to (similar to 2mm) the orifice of a mass spectrometer applied with a high voltage. Taylor cone was observed from the sample droplet, and a spray emitted from the cone apex was generated. The analyte ion signals derived from the droplet were obtained by the mass spectrometer. The ionization process is similar to that in ESI although no direct electric contact was applied on the sample site. The sample droplet polarized by the high electric field provided by the mass spectrometer initiated the ionization process. The dielectric sample loading substrate facilitated further the polarization process, resulting in the formation of Taylor cone. The mass spectral profiles obtained via this approach resembled those obtained using ESI-MS. Multiply charged ions dominated the mass spectra of peptides and proteins, whereas singly charged ions dominated the mass spectra of small molecules such as amino acids and small organic molecules. In addition to liquid samples, this approach can be used for the analysis of solid and viscous samples. A small droplet containing suitable solvent (5-10 mu l) was directly deposited on the surface of the solid (or viscous) sample, placed close the orifice of mass spectrometer applied with a high voltage. Taylor cone derived from the droplet was immediately formed followed by electrospray processes to generate gas-phase ions for MS analysis. Analyte ions derived from the main ingredients of pharmaceutical tablets and viscous ointment can be extracted into the solvent droplet in situ and observed using a mass spectrometer. Copyright (c) 2015 John Wiley & Sons, Ltd.
机译：在这项研究中，开发了一种极化诱导电喷雾电离质谱（ESI-MS）。将微尺寸的样品滴沉积在自然可用的电介质基板（例如水果或石头）上，然后将其放置在接近施加高压的质谱仪孔口（接近2mm）的位置。从样品液滴中观察到泰勒锥，并且产生了从锥顶点发射的喷雾。从液滴得到的分析物离子信号通过质谱仪获得。尽管没有直接电接触施加到样品位置，但电离过程与ESI中的电离过程相似。被质谱仪提供的高电场极化的样品滴引发了电离过程。加载电介质样品的基板进一步促进了极化过程，导致形成泰勒锥。通过这种方法获得的质谱图类似于使用ESI-MS获得的质谱图。带电荷的离子主导着肽和蛋白质的质谱，而带电荷的离子主导着小分子（例如氨基酸和有机小分子）的质谱。除液体样品外，该方法还可用于分析固体和粘性样品。将包含合适溶剂（5-10μl）的小滴直接沉积在固体（或粘性）样品的表面上，靠近高压施加的质谱仪孔口。立即形成源自液滴的泰勒锥，然后进行电喷雾工艺以生成气相离子用于MS分析。可以将来源于药物片剂和粘性软膏主要成分的分析离子原位提取到溶剂滴中，并使用质谱仪进行观察。版权所有（c）2015 John Wiley＆Sons，Ltd.

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