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首页> 外文期刊>Journal of mass spectrometry: JMS >Surface acoustic wave nebulization device with dual interdigitated transducers improves SAWN-MS performance
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Surface acoustic wave nebulization device with dual interdigitated transducers improves SAWN-MS performance

机译：具有双指状换能器的表面声波雾化设备提高了SAWN-MS性能

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摘要

We compared mass spectrometric (MS) performance of surface acoustic wave nebulization (SAWN) generated by a single interdigitated transducer (IDT) designed to produce a progressive wave (PW) to one with a dual IDT that can in theory generate standing waves (SW). Given that devices using dual IDTs had been shown to produce fewer large size droplets on average, we hypothesized they would improve MS performance by improving the efficiency of desolvation. Indeed, the SW-SAWN chip provided an improved limit of detection of 1femtomole of peptide placed on chip making it 100x more sensitive than the PW design. However, as measured by high-speed image recording and phase Doppler particle analyzer measurements, there was only a 26% increase in the small diameter (1-10 mu m) droplets produced from the new device, precluding a conclusion that the decrease in droplet size was solely responsible for the improvement in MS signaloise. Given that the dual IDT design produced a more instantaneous plume than the PW design, the more likely contributor to improved MS signaloise was concluded to be a higher ion flux entering the mass spectrometer for the dual IDT designs. Notably, the dual IDT device allowed production of much higher quality protein mass spectra up to about 20kDa, compared with the single IDT device. Copyright (c) 2016 John Wiley & Sons, Ltd.
机译：我们比较了由单个叉指式换能器（IDT）产生的表面声波雾化（SAWN）的质谱（MS）性能，该换能器设计用于产生渐进波（PW），而具有双IDT的理论上可以在理论上产生驻波（SW） 。考虑到使用双IDT的设备平均显示出较少的大尺寸液滴，我们假设它们将通过提高去溶剂化效率来改善MS性能。实际上，SW-SAWN芯片提供了对放置在芯片上的1femtomole肽的检测极限的改进，使其灵敏度比PW设计高100倍。但是，通过高速图像记录和相位多普勒粒子分析仪的测量，新设备产生的小直径（1-10微米）液滴的数量仅增加了26％，从而得出结论：液滴的减少尺寸是MS信号/噪声改善的唯一原因。考虑到双IDT设计产生的瞬时羽流比PW设计的瞬时羽流更多，因此可以得出结论，改进的MS信号/噪声的更可能的贡献是进入IDT双设计的质谱仪的离子通量更高。值得注意的是，与单IDT设备相比，双IDT设备可以产生高达约20kDa的更高质量的蛋白质质谱图。版权所有（c）2016 John Wiley＆Sons，Ltd.

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