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首页> 外文期刊>Journal of mass spectrometry: JMS >Application of porous metal enrichment probe sampling to single cell analysis using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS)
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Application of porous metal enrichment probe sampling to single cell analysis using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS)

机译：多孔金属富集探针采样在基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）的单细胞分析中的应用

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摘要

There is an increasing need for analyzing metabolism in a single cell, which is important to understand the nature of cellular heterogeneity, disease, growth and specialization, etc. However, single cell analysis is often challenging for the traces of samples. In the present study, porous metal enrichment probe sampling combined with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) has been applied for in situ analysis of live onion epidemic cell. Porous probe, treated by corroding copper wire with HCl, was directly inserted into a single cell to get cell solution. A self-made linear actuator was enough to control the penetration of probe into the target cell accurately. Then samples on the tip of probe were eluted and detected by a commercial MALDI-TOF-MS directly. The formation of porous microstructure on the probe surface increased the adsorptive capacity of cell solution. The sensitivity of porous probe sampling was 6 times higher than uncorroded probes generally. This method provides a sensitive and convenient way for the sampling and detection of single cell solution. Copyright (c) 2015 John Wiley & Sons, Ltd.
机译：越来越需要分析单个细胞中的代谢，这对于理解细胞异质性，疾病，生长和特化等的性质非常重要。但是，单细胞分析对于样品的痕迹通常具有挑战性。在本研究中，多孔金属富集探针采样结合基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）已用于活洋葱流行细胞的原位分析。通过用HCl腐蚀铜线处理的多孔探针直接插入单个细胞中，以得到细胞溶液。自制的线性执行器足以控制探针准确地刺入目标细胞。然后洗脱探针尖端的样品，并直接通过商业MALDI-TOF-MS检测。探针表面上的多孔微结构的形成增加了细胞溶液的吸附能力。多孔探针采样的灵敏度通常是未腐蚀探针的6倍。该方法为单细胞溶液的采样和检测提供了灵敏而方便的方法。版权所有（c）2015 John Wiley＆Sons，Ltd.

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