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首页> 外文期刊>Journal of mass spectrometry: JMS >The use of matrix coating assisted by an electric field (MCAEF) to enhance mass spectrometric imaging of human prostate cancer biomarkers
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The use of matrix coating assisted by an electric field (MCAEF) to enhance mass spectrometric imaging of human prostate cancer biomarkers

机译：在电场辅助下使用基质涂层（MCAEF）增强人类前列腺癌生物标志物的质谱成像

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摘要

In this work, we combined a newly developed matrix coating technique - matrix coating assisted by an electric field (MCAEF) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to enhance the imaging of peptides and proteins in tissue specimens of human prostate cancer. MCAEF increased the signal-to-noise ratios of the detected proteins by a factor of 2 to 5, and 232 signals were detected within the m/z 3500-37500 mass range on a time-of-flight mass spectrometer and with the sinapinic acid MALDI matrix. Among these species, three proteins (S100-A9, S100-A10, and S100-A12) were only observed in the cancerous cell region and 14 proteins, including a fragment of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase kinase 2, a fragment of cAMP-regulated phosphoprotein 19, 3 apolipoproteins (C-I, A-I, and A-II), 2S100 proteins (A6 and A8), -microseminoprotein, tumor protein D52, -1-acid glycoprotein 1, heat shock protein -1, prostate-specific antigen, and 2 unidentified large peptides at m/z 5002.2 and 6704.2, showed significantly differential distributions at the p<0.05 (t-test) level between the cancerous and the noncancerous regions of the tissue. Among these 17 species, the distributions of apolipoprotein C-I, S100-A6, and S100-A8 were verified by immunohistological staining. In summary, this study resulted in the imaging of the largest group of proteins in prostate cancer tissues by MALDI-MS reported thus far, and is the first to show a correlation between S100 proteins and prostate cancer in a MS imaging study. The successful imaging of the three proteins only found in the cancerous tissues, as well as those showing differential expressions demonstrated the potential of MCAEF-MALDI/MS for the in situ detection of potential cancer biomarkers. Copyright (c) 2015 John Wiley & Sons, Ltd.
机译：在这项工作中，我们结合了新开发的基质涂层技术-借助电场（MCAEF）辅助的基质涂层和基质辅助的激光解吸/电离质谱（MALDI-MS），以增强组织标本中肽和蛋白质的成像人类前列腺癌。 MCAEF将检测到的蛋白质的信噪比提高了2到5倍，并且在飞行时间质谱仪上和使用芥子酸在m / z 3500-37500质量范围内检测到232个信号MALDI矩阵。在这些物种中，仅在癌细胞区域中观察到三种蛋白质（S100-A9，S100-A10和S100-A12）和14种蛋白质，包括促分裂原活化蛋白激酶/细胞外信号调节激酶激酶2的片段，cAMP调节的磷蛋白19、3种载脂蛋白（CI，AI和A-II），2S100蛋白（A6和A8），-微丝蛋白，肿瘤蛋白D52，-1酸糖蛋白1，热休克蛋白-1的片段，前列腺特异性抗原和m / z 5002.2和6704.2处的2种未鉴定的大肽在组织的癌性和非癌性区域之间的p <0.05（t-test）水平上显示出明显的差异分布。在这17个物种中，通过免疫组织学染色证实了载脂蛋白C-1，S100-A6和S100-A8的分布。总而言之，这项研究导致迄今为止报道的MALDI-MS对前列腺癌组织中最大的一组蛋白质成像，并且是首次在MS成像研究中显示S100蛋白与前列腺癌之间的相关性。仅在癌组织中发现的三种蛋白质以及显示出差异表达的三种蛋白质的成功成像，证明了MCAEF-MALDI / MS在原位检测潜在癌症生物标志物的潜力。版权所有（c）2015 John Wiley＆Sons，Ltd.

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