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首页> 外文期刊>Journal of mass spectrometry: JMS >Separation and identification of purine nucleosides in the urine of patients with malignant cancer by reverse phase liquid chromatography/electrospray tandem mass spectrometry
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Separation and identification of purine nucleosides in the urine of patients with malignant cancer by reverse phase liquid chromatography/electrospray tandem mass spectrometry

机译：反相液相色谱/电喷雾串联质谱法对恶性肿瘤患者尿液中嘌呤核苷的分离与鉴定

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摘要

Urinary-modified nucleosides have a potential role as cancer biomarkers for a number of malignant diseases. High performance liquid chromatography (HPLC) was combined with full-scan mass spectrometry, MS/MS analysis and accurate mass measurements in order to identify purine nucleosides purified from urine. Potential purine nucleosides were assessed by their evident UV absorbance in the HPLC chromatogram and then further examined by the mass spectrometric techniques. In this manner, numerous modified purine nucleosides were identified in the urine samples from cancer patients including xanthine, adenosine, N1-methyladenosine, 5'-deoxy-5'-methylthioadenosine, 2-methyladenosine, N6-threonylcarbamoyladenosine, inosine, N1-methylinosine, guanosine,N1-methylguanosine, N7-methylguanine, N2-methylguanosine, N2,N2-dimethyguanosine, N2,N2,N7-trimethylguanosine. Furthermore, a number of novel purine nucleosides were tentatively identified via critical interpretation of the combined mass spectrometric data including N3-methyladenosine, N7-methyladenine, 5'-dehydro-2'-deoxyinosine, N3-methylguanine, O6-methylguanosine, N1,N2,N7-trimethylguanosine, N7-methyl-N2-ethylguanosine and N7-methyl-N1-ethylguanosine.
机译：尿液修饰的核苷具有作为许多恶性疾病的癌症生物标记物的潜在作用。高效液相色谱（HPLC）与全扫描质谱，MS / MS分析和准确的质量测量相结合，以鉴定从尿液中纯化的嘌呤核苷。潜在的嘌呤核苷通过HPLC色谱图中明显的UV吸收度进行评估，然后通过质谱技术进一步检查。以这种方式，在癌症患者的尿液样品中鉴定出许多修饰的嘌呤核苷，包括黄嘌呤，腺苷，N1-甲基腺苷，5'-脱氧-5'-甲基硫代腺苷，2-甲基腺苷，N6-苏氨酸氨基甲酰腺苷，肌苷，N1-甲基肌苷，鸟苷，N1-甲基鸟嘌呤，N7-甲基鸟嘌呤，N2-甲基鸟嘌呤，N2，N2-二甲基鸟嘌呤，N2，N2，N7-三甲基鸟嘌呤。此外，通过对组合质谱数据的批判性解释，初步确定了许多新的嘌呤核苷，包括N3-甲基腺苷，N7-甲基腺嘌呤，5'-脱氢-2'-脱氧肌苷，N3-甲基鸟嘌呤，O6-甲基鸟苷，N1，N2 N7-三甲基鸟苷，N7-甲基-N2-乙基鸟苷和N7-甲基-N1-乙基鸟苷。

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