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首页> 外文期刊>Journal of mass spectrometry: JMS >Microfluidic chips for mass spectrometry-based proteomics
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Microfluidic chips for mass spectrometry-based proteomics

机译：用于基于质谱的蛋白质组学的微流控芯片

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摘要

Microfluidic devices coupled to mass spectrometers have emerged as excellent tools for solving the complex analytical challenges associated with the field of proteomics. Current proteome identification procedures are accomplished through a series of steps that require many hours of labor-intensive work. Microfluidics can play an important role in proteomic sample preparation steps prior to mass spectral identification such as sample cleanup, digestion, and separations due to its ability to handle small sample quantities with the potential for high-throughput parallel analysis. To utilize microfluidic devices for proteomic analysis, an efficient interface between the microchip and the mass spectrometer is required. This tutorial provides an overview of the technologies and applications of microfluidic chips coupled to mass spectrometry for proteome analysis. Various approaches for combining microfluidic devices with electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) are summarized and applications of chip-based separations and digestion technologies to proteomic analysis are presented.
机译：结合质谱仪的微流控设备已经成为解决蛋白质组学领域复杂分析难题的优秀工具。当前的蛋白质组鉴定程序是通过一系列步骤来完成的，这些步骤需要许多小时的劳动强度工作。由于微流体能够处理少量样品并具有高通量并行分析的潜力，因此在质谱鉴定之前的蛋白质组学样品制备步骤（例如样品净化，消化和分离）中可发挥重要作用。为了利用微流体装置进行蛋白质组学分析，需要微芯片和质谱仪之间的有效接口。本教程概述了与蛋白质组分析相结合的微流控芯片技术和应用。总结了将微流控设备与电喷雾电离（ESI）和基质辅助激光解吸/电离（MALDI）结合的各种方法，并介绍了基于芯片的分离和消化技术在蛋白质组学分析中的应用。

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