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首页> 外文期刊>Journal of mass spectrometry: JMS >2-(2-Aminoethylamino)-5-nitropyridine as a basic matrix for negative-mode matrix-assisted laser desorption/ionization analysis of phospholipids
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2-(2-Aminoethylamino)-5-nitropyridine as a basic matrix for negative-mode matrix-assisted laser desorption/ionization analysis of phospholipids

机译：2-（2-氨基乙基氨基）-5-硝基吡啶作为基本模型，用于磷脂的负模基质辅助激光解吸/电离分析

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摘要

Fast and easy analysis of phospholipids (PLs) by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been well demonstrated. However, when using common organic matrices, such as 2,5-dihydroxybenzoic acid (DHB), the detection of most PL classes in positive-ion mode is difficult when PLs containing zwitterionic groups, such as phosphatidylcholines (PCs) and sphingomyelins (SMs) are present. To reduce this limitation, 2-(2-aminoethyloamino)5-nitropyridine (AAN), a basic compound, was evaluated as an alternative matrix. Negative-ion spectra showed enhanced detection of phosphatidyl ethanolamines (PEs), phosphatidyl serines (PSs), phosphatidyl glycerols (PGs), and phosphatidyl inositols (PIs) in simple mixtures and in a crude methanolic soybean extract. The relative ionization efficiency (RIE) was highest for Pis and lowest for PGs, PSs, and PEs. Compared to DHB and para-nitroaniline, AAN resulted in greater sensitivity for the detection of PL classes in the negative mode. Indeed, the S/N ratio was nearly an order of magnitude higher than that reported for similar PI concentrations but with DHB. MALDI spots produced with AAN were homogeneous thus allowing automation and improved reproducibility. Positive-mode traces could also be acquired with AAN as the matrix, but with lower sensitivity than in the negative mode.
机译：通过基质辅助激光解吸/电离质谱（MALDI-MS）可以快速，轻松地分析磷脂（PLs）。但是，当使用常见的有机基质（例如2,5-二羟基苯甲酸（DHB））时，当包含两性离子基团的PL（例如磷脂酰胆碱（PC）和鞘磷脂（SM））时，很难以阳离子模式检测大多数PL类。存在。为了减少此限制，评估了碱性化合物2-（2-氨基乙基氨基）5-硝基吡啶（AAN）作为替代基质。负离子光谱显示，在简单的混合物和粗制甲醇大豆提取物中，对磷脂酰乙醇胺（PEs），磷脂酰丝氨酸（PSs），磷脂酰甘油（PGs）和磷脂酰肌醇（PIs）的检测得到增强。 Pi的相对电离效率（RIE）最高，PG，PS和PE的相对电离效率最低。与DHB和对硝基苯胺相比，AAN在阴性模式下检测PL类的灵敏度更高。实际上，信噪比比报道的类似PI浓度但带有DHB的信噪比高出近一个数量级。用AAN生产的MALDI点是均匀的，因此可以实现自动化并提高重现性。也可以使用AAN作为矩阵来获取正模式迹线，但灵敏度要低于负模式。

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