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首页> 外文期刊>Journal of mass spectrometry: JMS >Unambiguous characterization and tissue localization of Pru P 3 peach allergen by electrospray mass spectrometry and MALDI imaging
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Unambiguous characterization and tissue localization of Pru P 3 peach allergen by electrospray mass spectrometry and MALDI imaging

机译：电喷雾质谱和MALDI成像对Pru P 3桃过敏原的明确表征和组织定位

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摘要

The lipid transfer protein (LTP), Pru p 3, has been identified as the major allergen present in peach, and its sequence obtained by direct amino acid sequencing has been previously reported. However, several sequences, obtained from c-DNA and available in databases, show differences among them and from the originally proposed structure. In this paper, we report the fast and unambiguous determination of the structure of Pru p 3 protein, extracted from three different varieties of peach, by electrospray ionization mass spectrometry (ESI-MS), both coupled to single stage (quadrupole) or advanced (FT-HRMS) analyzers. The structure was identical to one of the cDNA-derived sequences and different in two positions from the previously reported structure obtained by amino acid sequencing. Moreover, the exclusive localization of the protein in the outer part of the fruits was assessed by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging (MALDI MSI). The results reported here demonstrate the full potential of mass spectrometry for rapidly obtaining high quality structural data of relevant food proteins.
机译：脂质转移蛋白（LTP）Pru p 3已被确定为桃子中存在的主要过敏原，先前已报道了通过直接氨基酸测序获得的序列。但是，从c-DNA获得并可以从数据库中获得的一些序列显示出它们之间以及与最初提出的结构之间的差异。在本文中，我们报告了通过电喷雾电离质谱（ESI-MS）快速，明确地确定了从三个不同品种的桃中提取的Pru p 3蛋白的结构，无论是单级质谱还是四级质谱FT-HRMS）分析仪。该结构与cDNA衍生序列之一相同，并且与先前报道的通过氨基酸测序获得的结构在两个位置不同。此外，通过基质辅助激光解吸电离质谱成像（MALDI MSI）评估了蛋白质在果实外部的唯一定位。此处报道的结果证明了质谱法在快速获得相关食品蛋白质的高质量结构数据方面的全部潜力。

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