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首页> 外文期刊>Journal of mass spectrometry: JMS >Allergenic compounds on the inner and outer surfaces of natural latex gloves: MALDI mass spectrometry and imaging of proteinous allergens
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Allergenic compounds on the inner and outer surfaces of natural latex gloves: MALDI mass spectrometry and imaging of proteinous allergens

机译：天然乳胶手套内外表面的致敏化合物：MALDI质谱和蛋白质过敏原成像

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摘要

Natural latex gloves are the cause of a severe health problem to an increasing number of healthcare workers or patients due to the presence of protein allergens as Hevein or Rubber Elongation Factor (REF). One of the most challenging problems is the in situ localization of theses allergens in, e.g. gloves, to estimate the allergenic potential of the latex material. A sample preparation protocol applying a binary matrix-assisted laser desorption/ionization(MALDI) matrix containing alpha-cyano-4-hydroxy cinnamic acid (CHCA) and 2,5-dihydroxy benzoic acid (DHB) on trifluoro acetic acid (TFA) etched latex glove surfaces allowed the direct determination (exact molecular weight) of Hevein, REF and a truncated form of REF (tREF) within nine different brands of natural latex gloves by means of MALDI-TOF-MS in the linear mode. MALDI mass spectrometry demonstrated that Hevein, tREF and REF were present on the inner surfaces (in direct contact with the skin) of many, but not all, investigated gloves without any prior extraction procedure. Additionally, different isoforms of the allergen Hevein were detected (exhibiting ragged C-termini). tREF and REF could always be detected beside each other, but were not observed on every latex glove sample, which contained Hevein. It was also demonstrated that there is a significant difference in terms of proteins and polymers between inner and outer surfaces of gloves, which helps to explain the different allergenic potential of these. MALDI imaging allowed for the first time the unambiguous localization of all three allergens in parallel and showed that Hevein was present on 36% of the investigated area of a latex glove with a certain localization, whereupon, tREF and REF were only found on 25% of the investigated material.
机译：天然乳胶手套由于存在蛋白质过敏原（如橡胶蛋白或橡胶伸长因子（REF）），对越来越多的医护人员或患者造成严重的健康问题。最具挑战性的问题之一是这些过敏原的原位定位，例如手套，以估计乳胶材料的致敏潜力。样品制备方案在蚀刻的三氟乙酸（TFA）上应用包含α-氰基-4-羟基肉桂酸（CHCA）和2,5-二羟基苯甲酸（DHB）的二元基质辅助激光解吸/电离（MALDI）基质乳胶手套表面允许通过线性模式的MALDI-TOF-MS在九种不同品牌的天然乳胶手套中直接测定（精确分子量）橡胶，REF和截短形式的REF（tREF）。 MALDI质谱法表明，许多（但不是全部）被研究的手套的内表面（与皮肤直接接触）中均存在Hevein，tREF和REF，而没有进行任何事先的提取程序。另外，还检测到变应原戊肝素的不同同工型（表现出衣衫C的C末端）。 tREF和REF总是可以并排检测到，但是在每个包含Hevein的乳胶手套样品上都没有观察到。还证明了在手套的内表面和外表面之间在蛋白质和聚合物方面存在显着差异，这有助于解释这些手套的不同致敏潜力。 MALDI成像首次实现了所有三种过敏原的平行明确定位，并显示在特定位置的乳胶手套研究区域中，有36％的区域存在橡胶蛋白，因此，仅在25％的乳胶手套中发现了tREF和REF。被调查的材料。

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