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首页> 外文期刊>Journal of mass spectrometry: JMS >Increased sequence coverage of thymine-rich oligodeoxynucleotides by infrared multiphoton dissociation compared to collision-induced dissociation
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Increased sequence coverage of thymine-rich oligodeoxynucleotides by infrared multiphoton dissociation compared to collision-induced dissociation

机译：与碰撞诱导的解离相比，红外多光子解离增加了富含胸腺嘧啶的寡脱氧核苷酸的序列覆盖范围

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摘要

Infrared multiphoton dissociation (IRMPD) of thymine-rich oligodeoxynucleotides in a linear ion-trap mass spectrometer affords far more extensive fragmentation than conventional collision-induced dissociation (CID). For oligodeoxynucleotides containing one non-thymine base, CID results primarily in cleavage on the 3′ side of the non-thymine nucleobase, whereas IRMPD results in cleavages between all the nucleobases and thus provides complete sequence coverage. Furthermore, for oligodeoxynucleotides containing a single non-thymine base, it is shown that the full series of diagnostic sequence ions observed in the IRMPD mass spectra arise from secondary dissociation of the two primary products formed from the initial cleavage site located next to the non-thymine base.
机译：线性离子阱质谱仪中富含胸腺嘧啶的寡脱氧核苷酸的红外多光子解离（IRMPD）比常规碰撞诱导解离（CID）提供了更广泛的碎片。对于含有一个非胸腺嘧啶碱基的寡脱氧核苷酸，CID主要导致在非胸腺嘧啶核苷碱基的3'侧裂解，而IRMPD导致所有核苷碱基之间的裂解，从而提供了完整的序列覆盖范围。此外，对于含有单个非胸腺嘧啶碱基的寡脱氧核苷酸，研究表明，在IRMPD质谱图中观察到的全系列诊断序列离子，是由位于非磷酸胸腺嘧啶核苷旁边的初始裂解位点形成的两个主要产物的二级解离产生的。胸腺嘧啶碱。

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