• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of mass spectrometry: JMS >Diamond-like carbon coated polymer-based targets in microscope slide format for MALDI mass spectrometry
【24h】

Diamond-like carbon coated polymer-based targets in microscope slide format for MALDI mass spectrometry

机译：显微镜载玻片形式的类金刚石碳涂层聚合物基靶材，用于MALDI质谱分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Since the introduction of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry 20 years ago,[1,2] much effort has been put into the development of sample supports or targets to meet the users’ requirements for higher sensitivity, better reproducibility, selective enrichment or on-target sample cleanup.[3] Krutchinsky et al. report the use of a compact disc (CD) as a MALDI target and found CDs to be a promising replacement for traditional MALDI targets.[4] More recently, a novel composite target consisting of a polymer backing (a digital versatile disc), molybdenum interlayer and a diamond-like carbon (DLC) finish was presented, and its applicability as a sample support for LDI mass spectrometry was shown.[5] Besides this application to detect small molecule analytes without the interference from MALDI matrices and their fragments as well as clusters, DLC is also a promising material for MALDI target surfaces. Because of the mechanical robustness, chemical inertness and biocompatibility of DLC,[6,7] these targets could also be used in those cases where analyte interaction (e.g. phosphopeptides) with metal surfaces or corrosive additives render classical stainless steel sample supports unusable. Additionally, the design of these novel targets and the techniques applied for their production would make it possible to use them as disposables, thereby eliminating the problems inherent in such cleaning procedures as used for standard metal targets like mechanical damage, residues of detergents, change of surface properties, carryover from previously prepared samples and the consumption of solvents and time.
机译：自20年前引入基质辅助激光解吸/电离（MALDI）质谱仪以来，[1,2]已投入大量精力开发样品支持物或目标物，以满足用户对更高灵敏度，更好重现性的要求，选择性富集或目标样品净化。[3]克拉钦斯基等。报告使用光盘作为MALDI目标，并发现CD有望取代传统的MALDI目标。[4]最近，有人提出了一种新型复合靶材，该复合靶材由聚合物背衬（数字多功能光盘），钼中间层和类金刚石碳（DLC）涂层组成，并显示了其作为LDI质谱样品支持的适用性。[5 ]除了用于检测小分子分析物而不受MALDI基质及其片段和簇干扰的应用之外，DLC还是MALDI目标表面的有前途的材料。由于DLC的机械强度，化学惰性和生物相容性，[6,7]这些目标也可用于那些分析物与金属表面或腐蚀性添加剂的相互作用（例如磷酸肽）使经典的不锈钢样品载体无法使用的情况。此外，这些新型靶材的设计及其生产中使用的技术将使其有可能用作一次性用品，从而消除了用于标准金属靶材的此类清洁程序所固有的问题，例如机械损伤，洗涤剂残留，清洁剂的变化等。表面特性，先前制备的样品的残留物以及溶剂的消耗和时间。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号