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首页> 外文期刊>Journal of mass spectrometry: JMS >Parental relationships among three grape varieties studied by MALDI of grape seed protein profiles
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Parental relationships among three grape varieties studied by MALDI of grape seed protein profiles

机译：用MALDI研究三种葡萄品种的亲本关系

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摘要

Two Raboso cultivars, i.e. Raboso Veronese and Raboso Piave, are two black Vitis vinifera varieties. A genetic study suggested that Raboso Veronese is the progeny of a spontaneous cross between Raboso Piave and Marzemina Bianca cultivars. Parental relationships are usually investigated by genetic studies, which are effective to establish genetic links among different cultivars. Considering that proteome is the genome expression, in this article we evaluated the power of seed protein profiles obtained by matrix-assisted laser desorption/ionization (MALDI)/MS for parentage investigation. The three cultivars lead to very similar spectra with differences in the relative intensity of the most abundant species and the presence of very weak specific ions. In order to evaluate the analytical significance of these aspects, the variability due to instrumental factors and due to different harvesting areas and years of the same cultivars have been considered and measured by the calculation of discrepancy factor values. On one hand, the results obtained can be considered a valid confirmation of the genomic findings, whereas on the other hand, the results provide evidence for the ability of MALDI/MS to individuate minor differences in protein profiles of complex protein mixtures.
机译：两种Raboso品种，即Raboso Veronese和Raboso Piave，是两个黑色的Vitis vinifera品种。一项遗传研究表明，Raboso Veronese是Raboso Piave和Marzemina Bianca品种之间自发杂交的后代。父母关系通常通过遗传研究进行调查，这可以有效地建立不同品种之间的遗传联系。考虑到蛋白质组是基因组表达，在本文中，我们评估了通过基质辅助激光解吸/电离（MALDI）/ MS获得的种子蛋白谱用于亲子鉴定的能力。这三个品种产生的光谱非常相似，但最丰富的物种的相对强度有所不同，并且存在非常弱的比离子。为了评估这些方面的分析意义，已经通过计算差异因子值来考虑和测量由于仪器因素以及由于相同品种的不同收割面积和年份造成的变异性。一方面，获得的结果可被视为对基因组发现的有效证实，而另一方面，结果为MALDI / MS区分复杂蛋白质混合物的蛋白质谱中微小差异的能力提供了证据。

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