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首页> 外文期刊>Journal of mass spectrometry: JMS >Multifunctional nanoparticles composite for MALDI-MS: Cd~(2+)-doped carbon nanotubes with CdS nanoparticles as the matrix, preconcentrating and accelerating probes of microwave enzymatic digestion of peptides and proteins for direct MALDI-MS analysis
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Multifunctional nanoparticles composite for MALDI-MS: Cd~(2+)-doped carbon nanotubes with CdS nanoparticles as the matrix, preconcentrating and accelerating probes of microwave enzymatic digestion of peptides and proteins for direct MALDI-MS analysis

机译：用于MALDI-MS的多功能纳米颗粒复合材料：以CdS纳米颗粒为基质的Cd〜（2+）掺杂碳纳米管，微波酶消化肽和蛋白质的预浓缩和加速探针，用于直接MALDI-MS分析

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For the first time, we utilized multifunctional nanoparticles composite (NPs composite) for matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) analysis of peptides and proteins. Multiwalled carbon nanotubes doped with Cd~(2+) ions and modified with cadmium sulfide NPs were synthesized by a chemical reduction method at room temperature. The multifunctional NPs composite applied for the analysis of peptides and microwave-digested proteins in the atmospheric pressure matrix-assisted laser desorption/ionization ion-trap and MALDI time-of-flight (TOF) mass spectrometry (MS) was successfully demonstrated. The maximum detection sensitivity for peptides in MALDI-MS was achieved by the adsorption of negatively charged peptides onto the surfaces of NP composite through electrostatic interactions. The optimal conditions of peptide mixtures were obtained at 20 min of incubation time using 1 mg of NPs composite when the pH of the sample solution was kept higher than the pI values of peptides. The potentiality of the NP composite in the preconcentration of peptides was compared with that of the individual NP by calculating the preconcentration factors (PF) and found that the NPs composite showed a 4-6 times of PF than the other NPs. In addition, the NPs composite was also applied as heat-absorbing materials for efficient microwave tryptic digestion of cytochrome c and lysozyme from milk protein in MALDI-TOF-MS analysis. We believe that the use of NPs composite technique would be an efficient and powerful preconcentrating tool for MALDI-MS for the study of proteome research.
机译：首次，我们利用多功能纳米颗粒复合物（NPs复合物）进行肽和蛋白质的基质辅助激光解吸/电离质谱（MALDI-MS）分析。在室温下通过化学还原法合成了掺杂有Cd〜（2+）离子和硫化镉纳米粒子修饰的多壁碳纳米管。成功地证明了该多功能纳米复合材料用于分析大气压基质辅助激光解吸/电离离子阱和MALDI飞行时间（TOF）质谱（MS）中的肽和微波消化的蛋白质。 MALDI-MS中肽段的最大检测灵敏度是通过静电相互作用将带负电荷的肽段吸附到NP复合材料的表面上而实现的。当样品溶液的pH值保持在高于肽段的pI值时，使用1 mg NPs复合物在20分钟的孵育时间内可获得肽段混合物的最佳条件。通过计算预浓缩因子（PF），将NP复合物在肽的预浓缩中的潜能与单个NP的潜能进行了比较，发现该NPs复合物的PF是其他NP的4-6倍。此外，NPs复合材料还被用作吸热材料，用于在MALDI-TOF-MS分析中有效地微波胰蛋白酶消化牛奶蛋白中的细胞色素c和溶菌酶。我们相信，NPs复合技术的使用将是用于蛋白质组学研究的MALDI-MS的高效强大的预浓缩工具。

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