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首页> 外文期刊>Journal of mass spectrometry: JMS >Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS)
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Detection of protein from detergent solutions by probe electrospray ionization mass spectrometry (PESI-MS)

机译：探针电喷雾电离质谱（PESI-MS）从洗涤剂溶液中检测蛋白质

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摘要

Detergents are necessarily used for different extraction protocols of proteins from biological cells or tissues. After the extraction, elimination of detergent is necessary for the better performance of electrospray ionization mass spectrometry (ESI-MS). Elimination of detergents is laborious and time-consuming, and also sample loss may be unavoidable. Probe electrospray ionization (PESI) developed in our laboratory has been found to be tolerant to the presence of salts and buffers in sample solutions. In this report, it was examined whether PESI is applicable to the sample solutions that contain high-concentration of detergents. It was found that PESI is highly tolerant to the presence of sodium dodecyl sulphate, cetyl trimethylamminium bromide, Triton X100 and 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate compared with conventional ESI and nanoESI. Therefore, PESI can be a potential analytical tool for direct analysis of protein extracts and digests containing high-concentration detergents.
机译：洗涤剂必须用于从生物细胞或组织中提取蛋白质的不同方案。提取后，必须使用去污剂才能获得更好的电喷雾电离质谱（ESI-MS）性能。消除去污剂既费力又费时，而且样品流失也不可避免。我们实验室开发的探针电喷雾电离（PESI）被发现可以耐受样品溶液中盐和缓冲液的存在。在此报告中，我们检查了PESI是否适用于含有高浓度清洁剂的样品溶液。已经发现，与常规的ESI和nanoESI相比，PESI对十二烷基硫酸钠，十六烷基三甲基溴化铵，Triton X100和3-[（（3-胆酰胺基丙基）二甲基铵基] -1-丙烷磺酸盐]的存在高度耐受。因此，PESI可能是直接分析包含高浓度清洁剂的蛋白质提取物和消化物的潜在分析工具。

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