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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Investigation of double stranded DNA damage induced by quercetin-copper(II) using piezoelectric quartz crystal impedance technique and potentiometric stripping analysis
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Investigation of double stranded DNA damage induced by quercetin-copper(II) using piezoelectric quartz crystal impedance technique and potentiometric stripping analysis

机译：槲皮素-铜（II）诱导的双链DNA损伤的压电石英晶体阻抗技术和电位剥离分析研究

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摘要

DNA damage by quercetin-CU2+ was monitored in real time by piezoelectric quartz crystal impedance (PQCI) technique. In the PQCI analysis, the frequency change was caused mainly by the changes in density-viscosity of DNA solution in the damage course. The influences of DNA, Cu2+, and quercetin concentrations on the motional resistance change (DeltaR(m).) were investigated in detail. The results showed that quercetin exhibited pro-oxidative damage at lower concentrations while anti-oxidative protection at higher concentrations, and DeltaR(m), exhibited a linear relationship in the DNA concentration range from 200 to 1600 mug/ml. Potentiometric. stripping analysis (PSA) was also used to observe the electrochemical behavior of damaged DNA. From PSA, a new peak at 0.84 V and a higher peak at 1.06 V were discovered, which suggested that more purines were exposed to the electrode surface during the damage course. In agarose-gel electrophoresis, catalase and biquinoline were found to effectively inhibit DNA damage, therefore, a possible damage mechanism was proposed. (C) 2004 Elsevier B.V. All rights reserved.
机译：通过压电石英晶体阻抗（PQCI）技术实时监测槲皮素-CU2 +对DNA的损伤。在PQCI分析中，频率变化主要是由于DNA溶液在损伤过程中密度-粘度的变化引起的。详细研究了DNA，Cu2 +和槲皮素浓度对运动阻力变化（DeltaR（m）。）的影响。结果表明，槲皮素在较低浓度下表现出促氧化损伤，而在较高浓度下表现出抗氧化保护作用，而DeltaR（m）在200至1600 mug / ml的DNA浓度范围内表现出线性关系。电位计。汽提分析（PSA）也用于观察受损DNA的电化学行为。从PSA中发现了一个新的峰，在0.84 V，一个更高的峰在1.06 V，这表明在损伤过程中更多的嘌呤暴露于电极表面。在琼脂糖凝胶电泳中，发现过氧化氢酶和联喹啉可有效抑制DNA损伤，因此，提出了可能的损伤机理。 （C）2004 Elsevier B.V.保留所有权利。

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