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首页> 外文期刊>Journal of marine systems: journal of the European Association of Marine Sciences and Techniques >Suspended marine particulate proteins in coastal and oligotrophic waters
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Suspended marine particulate proteins in coastal and oligotrophic waters

机译：沿海和贫营养水域中悬浮的海洋颗粒蛋白

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Metaproteomic analyses were performed on suspended sediments collected in one coastal environment (Washington margin, Pacific Ocean, n = 5) and two oligotrophic environments (Atlantic Ocean near BATS, n = 5, and Pacific Ocean near HOTS, n = 5). Using a database of 2.3 million marine proteins developed using the NCBI database, 443 unique peptides were detected from which 363 unique proteins were identified. Samples from the euphotic zone contained on average 2-3x more identifiable proteins than deeper waters (150-1500 m) and these proteins were predominately from photosynthetic organisms. Diatom peptides dominate the spectra of the Washington margin while peptides from cyanobacteria, such as Synechococcus sp. dominated the spectra of both oligotrophic sites. Despite differences in the exact proteins identified at each location, there is good agreement for protein function and cellular location. Proteins in surface waters code for a variety of cellular functions including photosynthesis (24% of detected proteins), energy production (10%), membrane production (9%) and genetic coding and reading (9%), and are split 60-40 between membrane proteins and intracellular cytoplasmic proteins. Sargasso Sea surface waters contain a suite of peptides consistent with proteins involved in circadian rhythms that promote both C and N fixation at night. At depth in the Sargasso Sea, both muscle-derived myosin protein and the muscle-hydrolyzing proteases deseasin MCP-01 and metalloprotease Mcp02 from gamma-proteobacteria were observed. Deeper waters contain peptides predominately sourced from gamma-proteobacteria (37% of detected proteins) and alpha-proteobacteria (26%), although peptides from membrane and photosynthetic proteins attributable to phytoplankton were still observed (13%). Relative to surface values, detection frequencies for bacterial membrane proteins and extracellular enzymes rose from 9 to 16 and 2 to 4% respectively below the thermocline and the overall balance between membrane proteins and intracellular proteins grows to an approximate 75-25 split. Unlike the phytoplankton membrane proteins, which are detrital in nature, the bacterial protein suite at depth is consistent with living biomass. (C) 2014 Elsevier B.V. All rights reserved.
机译：对在一个沿海环境（华盛顿边缘，太平洋，n = 5）和两个贫营养环境（BATS附近的大西洋，n = 5，HOTS附近的太平洋，n = 5）中收集的悬浮沉积物进行了元蛋白质组学分析。使用使用NCBI数据库开发的230万种海洋蛋白质的数据库，检测到443种独特的肽，从中鉴定出363种独特的蛋白质。与较深水域（150-1500 m）相比，来自富营养区的样品所含的可识别蛋白质平均要多2至3倍，这些蛋白质主要来自光合生物。硅藻肽在华盛顿边界的光谱中占主导地位，而蓝细菌的肽，例如Synechococcus sp。主导了两个贫营养位的光谱。尽管在每个位置鉴定出的确切蛋白质存在差异，但是对于蛋白质功能和细胞位置已经达成了很好的共识。地表水中的蛋白质编码多种细胞功能，包括光合作用（占检测蛋白质的24％），能量产生（10％），膜产生（9％）和遗传编码和读取（9％），并被分解为60-40在膜蛋白和细胞内胞质蛋白之间。 Sargasso海表水含有一组与昼夜节律有关的蛋白质一致的肽，这些蛋白质在晚上促进C和N固定。在马尾藻海的深处，观察到了肌肉来源的肌球蛋白和肌肉水解蛋白酶deseasin MCP-01和金属蛋白酶Mcp02。尽管仍观察到来自浮游植物的膜和光合蛋白的肽（13％），但较深的水含有主要来自γ-蛋白细菌（37％的检测蛋白）和α-蛋白细菌（26％）的肽。相对于表面值，细菌膜蛋白和细胞外酶的检测频率分别比温跃线低9％至16％和2％至4％，并且膜蛋白和细胞内蛋白之间的总体平衡增长到大约75-25。不同于浮游植物膜蛋白，其本质上是有害的，其细菌蛋白在深度上与活生物质一致。 （C）2014 Elsevier B.V.保留所有权利。

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