...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Medical Entomology >Identification of mosquito bloodmeals using polymerase chain reaction (PCR) with order-specific primers
【24h】

Identification of mosquito bloodmeals using polymerase chain reaction (PCR) with order-specific primers

机译：使用聚合酶链反应（PCR）和特定顺序引物鉴定蚊子的血粉

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A polymerase chain reaction (PCR) protocol was developed to identify host blood-meals from mosquitoes. Primers for the cytochrome b gene were designed to distinguish between mammalian and avian bloodmeals and further differentiate among four avian orders: passeriformes, falconiformes, columbiformes, and galliformes. The assay was validated by testing tissues from 18 species of passeriformes, three species of falconiformes, three species of columbiformes, and two species of galliformes. American crows were distinguished from other passeriformes by restriction enzyme digestion. Host bloodmeals from engorged mosquitoes collected in New York State were identified to avian order level. PCR was able to detect the mosquito bloodmeal for up to 3 d after feeding on a quail. Significantly, these studies use order-specific primers in a single PCR test to identify mosquito bloodmeals.
机译：开发了聚合酶链反应（PCR）方案以鉴定蚊子中的宿主血粉。细胞色素b基因的引物旨在区分哺乳动物和禽类的血粉，并进一步区分四个禽类：Passeriformes，Falconiformes，columbiformes和Galliformes。通过测试来自18种Passeriformes，3种Falconiformes，3种Columbiformes和2种Galliformes的组织来验证该试验的有效性。通过限制酶消化，美洲乌鸦与其他Passeriformes有所区别。来自纽约州的饱水蚊子的宿主血粉被鉴定为禽类。以鹌鹑为食后，PCR能够检测出蚊子血粉长达3天。重要的是，这些研究在单个PCR测试中使用了特定顺序的引物来鉴定蚊子的血粉。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号