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首页> 外文期刊>Journal of Medical Entomology >Genetic variability in geographical populations of Lutzomyia whitmani elucidated by RAPD-PCR
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Genetic variability in geographical populations of Lutzomyia whitmani elucidated by RAPD-PCR

机译：RAPD-PCR阐明惠氏梭菌地理种群的遗传变异

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摘要

Unequivocal identification of phlebotomine sand flies is of crucial importance in epidemiological studies of leishmaniasis, because certain species may act as vectors, depending on behavior and physiology. For Lutzomyia whitmani, a major vector of American human cutaneous leishmaniasis in Brazil, in increasing number of studies have suggested the existence of a species complex. In the present work, we evaluated the genetic variability of L. whitmani populations from four Brazilian foci of that disease: Corte de Pedra, Ilheus, Martinho Campos, and Serra de Baturite. Computational analysis of 85 characters, generated by RAPD-polymerase chain reaction, demonstrated high intrapopulational variability. Those characters led to sex discrimination in three of the populations, with the exception of Martinho Campos individuals, in which sex distinction was not complete. One and two interpopulational phenograms were obtained for females and males, respectively. A higher similarity was observed among the specimens from Ilheus, Corte de Pedra, and Serra de Baturite., whereas the Martinho Campos population remained external to that cluster. These results, which are in partial accordance with a previous morphometric survey of L. whitmani from the same regions, provide additional evidence to support the existence of at least two Spatial Clusters of biogeographical populations of L. whitmani in Brazil.
机译：在利什曼病的流行病学研究中毫不含糊地确定le虫的sand蝇是至关重要的，因为取决于行为和生理学，某些物种可能充当媒介。对于惠氏菌（Lutzomyia whitmani）（美国在巴西的人类皮肤利什曼病的主要传播媒介），越来越多的研究表明存在物种复合体。在当前的工作中，我们评估了来自四个巴西疫源地惠特曼乳杆菌种群的遗传变异性：Corte de Pedra，Ilheus，Martinho Campos和Serra de Baturite。 RAPD聚合酶链反应产生的85个字符的计算分析表明，种群内变异性很高。这些特征导致了三个人口中的性别歧视，但马丁尼奥·坎波斯（Martinho Campos）个人除外，他们的性别区分不完全。女性和男性分别获得了一个和两个族群间的关系图。在Ilheus，Corte de Pedra和Serra de Baturite的标本中观察到更高的相似性，而Martinho Campos种群仍在该簇的外部。这些结果部分与先前来自同一地区的惠特曼乳杆菌的形态测量结果部分相符，提供了额外的证据来支持巴西惠特曼乳杆菌生物地理种群的至少两个空间簇的存在。

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