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首页> 外文期刊>Journal of Medical Entomology >Light deprivation affects larval development and arrestin gene expression in Anopheles stephensi.
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Light deprivation affects larval development and arrestin gene expression in Anopheles stephensi.

机译:轻度剥夺会影响斯蒂芬按蚊的幼虫发育和restarin基因表达。

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摘要

The role of light exposure on the final stages of development of Anopheles stephensi larvae to pupae and adult mosquitoes was explored. We demonstrated a significant reduction in the development of adult mosquitoes when larvae were bred in the absence of light compared with the control group bred in alternating 12 h of light and 12 h of dark. To correlate these findings at the molecular level, RNA levels of the visual arrestin gene were examined. Arrestins are an important gene family that play a role in the vectorial capacity of Anopheles and mediate neurotransmission as well as olfactory and visual sensory reception in insects. Semiquantitative polymerase chain reaction showed a reduction in the expression of the visual arrestin gene in pupae that developed from larvae in the absence of light compared with larvae bred under normal conditions..
机译:探索了光照在斯蒂芬按蚊幼虫发育成p和成年蚊子的最后阶段中的作用。我们证明,与在光亮的12小时和黑暗的12小时交替饲养的对照组相比,在没有光照的情况下繁殖幼虫时,成年蚊子的发育显着减少。为了在分子水平上关联这些发现,检查了视觉抑制蛋白基因的RNA水平。抑制蛋白是重要的基因家族,在昆虫按蚊的媒介能力中起作用,并介导昆虫的神经传递以及嗅觉和视觉感觉的接收。与正常条件下繁殖的幼虫相比,半定量聚合酶链反应显示了在没有光照的情况下由幼虫发育而来的p中视觉抑制蛋白基因表达的减少。

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