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首页> 外文期刊>Journal of Medical Entomology >Molecular diagnosis of pyrethroid resistance in Mexican strains of Boophilus microplus (Acari : Ixodidae)
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Molecular diagnosis of pyrethroid resistance in Mexican strains of Boophilus microplus (Acari : Ixodidae)

机译：墨西哥Boophilus microplus（Acari：Ixodidae）菌株对拟除虫菊酯抗药性的分子诊断

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摘要

Polymerase chain reaction (PCR) diagnostic assays were used to identify possible resistance-associated roles of two amino acid substitutions found in pyrethroid resistance-associated genes of Boophilus microplus (Canestrini). Individual larvae from the San Felipe target site resistant strain and the Coatzacoalcos (Cz) metabolic resistant strain were separated into resistant and susceptible groups by larval packet bioassays and analyzed by PCR. A Phe --> Ile amino acid mutation in the sodium channel gene S6 transmembrane segment of domain III was found to have a close association with survival of acaricide treatments containing as high as 30% permethrin. As the permethrin close was increased, an increase was seen in the proportion of surviving larvae that possessed two mutated sodium channel alleles. An Asp --> Asn amino acid Substitution, originally found in high allele frequency in alleles of the CzEst9 esterase of the Cz strain, appeared to provide some resistance to permethrin. However, the presence of the mutation did not associate with resistance in the dose-response fashion seen with the sodium channel amino acid mutation. Resistance provided by CzEst9 might be more dependent on concentration of CzEst9 more so than the presence of a mutated allele.
机译：聚合酶链反应（PCR）诊断测定用于鉴定在Boophilus microplus（Canestrini）的拟除虫菊酯抗性相关基因中发现的两个氨基酸取代的可能抗性相关作用。通过幼虫小包生物测定法将来自San Felipe目标位点抗性菌株和Coatzacoalcos（Cz）代谢抗性菌株的幼虫分为抗性和易感组，并通过PCR进行分析。发现结构域III的钠通道基因S6跨膜区段中的Phe-> Ile氨基酸突变与含有高达30％的苄氯菊酯的杀螨剂治疗的存活率密切相关。随着苄氯菊酯封闭度的增加，具有两个突变的钠通道等位基因的存活幼虫的比例增加。 Asp-> Asn氨基酸取代原本是在Cz菌株CzEst9酯酶等位基因的等位基因中以高等位基因频率出现的，似乎提供了对氯菊酯的抗性。然而，该突变的存在与钠通道氨基酸突变所见的剂量反应方式与耐药性无关。 CzEst9提供的抗性可能比突变等位基因的存在更依赖于CzEst9的浓度。

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