首页> 外文期刊>Journal of Medical Entomology >Antibacterial hemoglobin fragments from the midgut of the soft tick, Ornithodoros moubata (Acari : Argasidae)
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Antibacterial hemoglobin fragments from the midgut of the soft tick, Ornithodoros moubata (Acari : Argasidae)

机译:来自软壁虱中肠Ornithodoros moubata(Acari:Argasidae)的抗菌血红蛋白片段

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摘要

Midgut contents of Ornithodoros monbata showed strong antibacterial activity against Staphylococcus aureus. A combination of reversed-phase chromatography and mass spectrometric analysis was used to isolate two antibacterial peptides from the tick midgut lumen. Partial amino acid sequences by Edman degradation of these two peptides showed they are identical with the 1-11 and 3-19 portions of rabbit a hemoglobin. Host rabbit a hemoglobin appears to be cleaved between Met(32) and Phe(33) to produce these two antibacterial peptides. Isolation of a host bovine hemoglobin fragment with antimicrobial activity has been demonstrated in the Ixodid tick, Boophilus microplus (Fogaca et al. 1999). Similar immune mechanisms in the two major families of ticks, Ixodidae and Argasidae, appear to use the hemoglobin of the host as an antimicrobial agent in midgut defense.
机译:鸟粪鸟粪中肠含量对金黄色葡萄球菌具有很强的抗菌活性。反相色谱和质谱分析的组合用于从壁虱中肠管腔中分离出两种抗菌肽。通过这两种肽的Edman降解,部分氨基酸序列显示它们与兔血红蛋白的1-11和3-19部分相同。宿主兔的血红蛋白似乎在Met(32)和Phe(33)之间裂解,从而产生这两种抗菌肽。具有抗微生物活性的宿主牛血红蛋白片段的分离已在艾克索迪克壁虱(Boophilus microplus)中得到证实(Fogaca等,1999)。 major科的两个主要家族的类似免疫机制似乎是利用宿主的血红蛋白作为中肠防御中的抗菌剂。

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