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首页> 外文期刊>Journal of Medical Entomology >Previous infection protects house finches from re-infection with St. Louis encephalitis virus
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Previous infection protects house finches from re-infection with St. Louis encephalitis virus

机译：先前的感染可保护雀科免受圣路易斯脑炎病毒的再次感染

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摘要

Antibody titers against St. Louis encephalitis virus (SLE) measured by a plaque reduction neutralization test (PRNT) decreased rapidly in house finches (Capodacus mexicanus) after initial infection, whereas antibodies measured by enzyme immunoassay (EIA) remained detectable in all birds for the length of the experiment, indicating long-term persistence and greater assay sensitivity of the EIA. After 52 wk, birds were challenged by subcutaneous inoculation with the same strain of SLE virus. Virus was not detected for 1-4 d postchallenge in blood samples tested by plaque assay and RT-PCR or by xenodiagnosis in Culex tarsalis fed concurrently and then held for 11 d at 26degreesC. Virus was detected by all three methods in control birds infected concurrently for the first time. Challenge with SLE produced a rapid and marked ananmestic rise in both neutralizing and EIA antibody titers that exceeded the primary response in the same birds or in concurrently inoculated control birds. At necropsy 4 wk postchallenge, 3 of 7 challenged and 1 of 2 positive control birds were chronically infected, with viral RNA detected by RT-PCR in brain, spleen, lung, and/or kidney tissues. Our results indicated that persistence of protective antibody prevents reinfection during the following season and may prevent the recrudescence of infectious virus in chronically infected birds.
机译：通过斑块减少中和试验（PRNT）测量的圣路易斯脑炎病毒（SLE）抗体滴度在初始感染后在室内雀（Capodacus mexicanus）中迅速下降，而通过酶免疫法（EIA）测量的抗体仍可在所有禽类中检测到实验的时间长度，表明EIA的长期持久性和更高的测定灵敏度。 52周后，通过皮下接种相同品系的SLE病毒攻击鸟类。在通过噬斑测定和RT-PCR或通过异种诊断在同时进食的库蚊（Culex tarsalis）测试的血液样本中，攻击后1-4天未检测到病毒，然后在26°C保持11天。通过三种方法在首次同时感染的对照禽中检测到病毒。用SLE进行攻击后，中和和EIA抗体滴度均迅速显着升高，这超出了同一只鸡或同时接种的对照鸡的主要反应。尸检后4周尸检，用RT-PCR检测到的病毒RNA在脑，脾，肺和/或肾组织中被慢性感染7只受攻击的小鼠中的3只和2只阳性对照鸡中的1只。我们的结果表明，保护性抗体的持久性可以防止在接下来的季节中再次感染，并且可以防止慢性感染禽类中传染性病毒的复发。

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