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首页> 外文期刊>Journal of Medical Entomology >Molecular evidence for novel tick-associated spotted fever group Rickettsiae from Thailand
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Molecular evidence for novel tick-associated spotted fever group Rickettsiae from Thailand

机译：来自泰国的新型与tick相关的斑点热组立克次体的分子证据

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摘要

Ticks are of considerable medical and veterinary importance because they directly harm the host through their feeding action and indirectly through vectoring many bacterial pathogens. Despite many ticks being known from Thailand, very little is known about the bacteria they may harbor. We report here the results of a survey of tick-associated bacteria in Thailand. A total of 334 individuals representing 14 species of ticks in five genera were collected from 10 locations in Thailand and were examined for the human pathogens, Borrelia, Francisella, Rickettsia, and the common arthropod endosymbionts, Wolbachia, by polymerase chain reaction (PCR) assay using specific primers. Rickettsial DNA was detected in 30% (9/30) of Amblyomma testudinarium (Koch, 1844) collected from Khao Yai National Park, Nakhon Nayok Province and 16.84% (16/95) of Hemaphysalis ornithophila (Hoogstraal and Kohls, 1959) collected from Khao Yai National Park, Nakhon Nayok Province and Khao Ang Rue Nai Wildlife Sanctuary, Chachoengsao Province. Rickettsiae DNA was not detected in any of the other tick species and no DNA of Borrelia, Francisella, or Wolbachia was detected in any of 14 tick species. Phylogenetic relationships among the rickettsiae detected in this study and those of other rickettsiae were inferred from comparison of sequences of the 17-kDa antigen gene, the citrate synthase gene (gltA), and the 190-kDa outer membrane protein gene (ompA). Results indicated that the three Thai rickettsiae detected in this study represent new rickettsiae genotypes and form a separate cluster among the spotted fever group rickettsiae.
机译：cks虫具有相当大的医学和兽医学重要性，因为虫通过其摄食作用直接伤害宿主，而间接传播许多细菌病原体则间接伤害宿主。尽管泰国知道许多s，但对它们可能藏匿的细菌知之甚少。我们在这里报告泰国tick相关细菌调查的结果。从泰国的10个地点收集了总共334个个体，分别代表5个属的14种壁虱，并通过聚合酶链反应（PCR）分析检测了人类病原体，疏螺旋体，弗朗西斯菌，立克次氏体和常见节肢动物内共生菌，沃尔巴克氏体。使用特定的引物。从那空那育府考艾国家公园收集的30％（9/30）盲肠睾丸中检出立克次体DNA（Koch，1844），从那曲纳鸟粪嗜性菌（Hoogstraal and Kohls，1959）收集到16.84％（16/95）的嗜血性嗜酸红细胞呵叻府（Nakhon Nayok Province）考艾国家公园（Khao Yai National Park）和北柳府考（Khao Ang Rue Nai）野生动物保护区。在其他任何壁虱物种中均未检测到立克次体DNA，在14种壁虱物种中均未检测到疏螺旋体，弗朗西斯菌或沃尔巴克氏体的DNA。通过比较17-kDa抗原基因，柠檬酸合酶基因（gltA）和190-kDa外膜蛋白基因（ompA）的序列，可以推断出本研究中发现的立克次体与其他立克次体之间的亲缘关系。结果表明，在该研究中检测到的三种泰国立克次体代表新的立克次体基因型，并在斑点发烧立克次体中形成了单独的簇。

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