• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Medical Entomology >Detection of novel Bartonella strains and Yersinia pestis in prairie dogs and their fleas (Siphonaptera : ceratophyllidae and pulicidae) using multiplex polymerase chain reaction
【24h】

Detection of novel Bartonella strains and Yersinia pestis in prairie dogs and their fleas (Siphonaptera : ceratophyllidae and pulicidae) using multiplex polymerase chain reaction

机译：多重聚合酶链反应检测草原犬鼠及其蚤中新的巴尔通体毒株和鼠疫耶尔森菌（Siphonaptera：ceratophyllidae和pulicidae）

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We developed a multiplex polymerase chain reaction (PCR) assay that simultaneously detects three types of flea-associated microorganisms. Targets for the assay were sequences encoding portions of the gltA, a 17-kDa antigen, and pla genes of Bartonella spp. Strong et al., Rickettsia spp. da Rocha-Lima, and Yersinia pestis Yersin, respectively. A total of 260 flea samples containing bloodmeal remnants were analyzed from fleas collected from abandoned prairie dog (Cynomys ludovicianus) burrows at the site of an active plague epizootic in Jefferson County, CO. Results indicated that 34 (13.1%) fleas were positive for Bartonella spp.,0 (0%) were positive for Rickettsia spp., and 120 (46.2%) were positive for Y. pestis. Twenty-three (8.8%) of these fleas were coinfected with Bartonella spp. and Y. pestis. A second group of 295 bloodmeal-containing fleas was collected and analyzed from abandoned burrows in Logan County, CO, where a prairie dog die-off had occurred 2-4 mo before the time of sampling. Of these 295 fleas, 7 (2.4%) were positive for Bartonella spp., 0 (0%) were positive for Rickettsia spp., and 46 (15.6%) were positive for Y pestis. Coinfections were not observed in fleas from the Logan County epizootic site. The multiplex PCR also was used to identify Y pestis and Bartonella in prairie dog blood and tissues. This report represents the first identification of Bartonella from prairie dogs and their fleas. Prairie dog fleas were tested with PCR, and the Bartonella PCR amplicons produced were sequenced and found to be closely related to similar sequences amplified from Bartonella that had been isolated from prairie dog blood samples. Phylogenetic analyses indicate that the sequences of bartonellae from prairie dogs and prairie dog fleas cluster tightly within a clade that is distinct from those containing other known Bartonella genotypes.
机译：我们开发了一种多重聚合酶链反应（PCR）分析方法，可同时检测三种类型的与跳蚤相关的微生物。该测定的靶标是编码gltA，17-kDa抗原和Bartonella spp的pla基因的部分的序列。 Strong等，立克次体属。 da Rocha-Lima和鼠疫耶尔森菌耶尔森。在科罗拉多州杰斐逊县一个活跃的鼠疫流行场所，从废弃的草原犬（Cynomys ludovicianus）洞穴收集的蚤中分析了总共260份含有血粉残留的蚤样品。结果表明，巴尔通体的蚤为34株（13.1％）阳性立克次体spp。，0（0％）为阳性，鼠疫耶尔森氏菌为120（46.2％）。这些跳蚤中有23（8.8％）与Bartonella spp共感染。和鼠疫耶尔森氏菌。从位于科罗拉多州洛根县的废弃洞穴中收集并分析了第二批含295种血粉的跳蚤，那里的土拨鼠死于采样前2-4个月。在这295个跳蚤中，巴尔通体属呈阳性的有7个（2.4％），立克次体属呈阳性的有0个（0％），鼠疫耶尔森氏菌呈阳性的有46个（15.6％）。在洛根县流行场所的跳蚤中未观察到合并感染。多重PCR还用于鉴定草原犬狗血液和组织中的鼠疫杆菌和巴尔通体。该报告代表了从草原犬鼠及其跳蚤中首次发现巴尔通体。用PCR测试了草原犬鼠的跳蚤，并对产生的Bartonella PCR扩增子进行了测序，发现与从草原犬鼠血液样本中分离的Bartonella扩增的相似序列密切相关。系统发育分析表明，草原犬鼠和草原犬鼠蚤中的巴尔通体序列紧密结合在进化枝中，该进化枝不同于包含其他已知巴尔通体基因型的进化枝。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号