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Role of bacteria in mediating the oviposition responses of Aedes albopictus (Diptera : Culicidae)

机译:细菌在介导白纹伊蚊的排卵反应中的作用

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摘要

The responses of Aedes albopictus to sources of oviposition attractants and stimulants were evaluated with a behavioral bioassay in which females attracted to odorants emanating from water were trapped on screens coated with an adhesive. Gravid mosquitoes were attracted to volatiles from larval-rearing water and soil-contaminated cotton towels. Bacteria were isolated from these substrates and from an organic infusion made with oak leaves. Through fatty acid-methyl ester analyses, six bacterial isolates from larval-rearing water, two isolates from soil-contaminated cotton towels, and three isolates from oak leaf infusion were identified to species. The response of gravid mosquitoes to these isolates was also evaluated in behavioral bioassays. Water containing Psychrobacter immobilis (from larval-rearing water), Sphingobacterium multivorum (from soil-contaminated cotton towels), and an undetermined Bacillus species (from oak leaf infusion) elicited significantly higher oviposition than control water without bacteria. Only volatiles collected from larval rearing water elicited significant electroantennogram responses in females.
机译:用行为生物测定法评估白纹伊蚊对产卵引诱剂和刺激物的反应,其中雌性被水吸引的增香剂所吸引,被困在涂有粘合剂的筛网上。饲养幼虫的水和被土壤污染的棉毛巾吸引了剧烈的蚊子挥发。从这些底物和用橡树叶制成的有机浸液中分离细菌。通过脂肪酸甲酯分析,从物种中鉴定出了六个来自幼虫饲养水中的细菌分离株,两个来自被土壤污染的棉毛巾的分离株以及三个来自橡木叶浸入的分离株。还通过行为生物测定法评估了妊娠蚊对这些分离株的反应。含有不动产Psychrobacter的水(来自幼虫饲养的水),Sphingobacterium multivorum(来自被土壤污染的棉毛巾)和不确定的芽孢杆菌属物种(来自橡木叶浸液)引起的排卵明显高于不含细菌的对照水。只有从幼体饲养水中收集到的挥发物才在雌性动物中引起显着的血管电图反应。

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