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首页> 外文期刊>Journal of Medical Entomology >Wicking assays for the rapid detection of West Nile and St. Louis encephalitis viral antigens in mosquitoes (Diptera : Culicidae)
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Wicking assays for the rapid detection of West Nile and St. Louis encephalitis viral antigens in mosquitoes (Diptera : Culicidae)

机译：快速检测蚊子中西尼罗河和圣路易斯脑炎病毒抗原的芯吸测定法（双翅目：：科）

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摘要

The recent outbreaks of West Nile (WN) encephalitis and St. Louis encephalitis (SLE) in the United States have highlighted the need for rapid and specific methods of detecting arboviral antigens in mosquitoes. We evaluated rapid, field-usable assays for detecting and differentiating WN and SLE viruses in mosquito pools, based on a patent-pending, inununochromatographic technology (VecTest) formatted on a dipstick. The device provides results in less than 20 min and can be used in laboratories with adequate containment facilities. In laboratory assessments, both the SLE and WN virus tests demonstrated sensitivity comparable with that of an antigen capture ELISA, but less than can be achieved with Vero cell plaque or reverse-transcriptase polymerase chain reaction assays. There was no evidence of cross-reaction when tested with high concentrations of heterologous flavivirus antigens or with Eastern equine encephalitis or Western equine encephalitis viruses. Both the WN and SLE dipstick tests delivered a clear positive result with a single positive specimen in a pool of 50 mosquitoes. This virus assay technology reduces the time required to obtain test results and will allow rapid medical threat assessment and effective targeting of vector control measures.
机译：在美国最近爆发的西尼罗河（WN）脑炎和圣路易斯脑炎（SLE）中，突显了对检测蚊子中虫媒病毒抗原的快速而特定方法的需求。我们基于在量油尺上格式化的一项正在申请专利的免疫色谱技术（VecTest），评估了快速，现场可用的检测方法，用于检测和区分蚊子池中的WN和SLE病毒。该设备可在不到20分钟的时间内提供结果，并且可在具有适当围堵设施的实验室中使用。在实验室评估中，SLE和WN病毒测试均显示出与抗原捕获ELISA相当的灵敏度，但低于Vero细胞噬菌斑或逆转录酶聚合酶链反应测定法所能达到的灵敏度。用高浓度的异源黄病毒抗原或东部马脑炎或西部马脑炎病毒进行测试时，没有交叉反应的证据。 WN和SLE量油尺测试均在50个蚊子池中以单个阳性标本提供了明显的阳性结果。这种病毒分析技术减少了获得测试结果所需的时间，并且可以进行快速的医学威胁评估和有效地控制媒介控制措施。

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