首页> 外文期刊>Journal of Medical Entomology >Analysis of the allergen expression of Blomia tropicalis and Blomia kulagini (Astigmata: Glycyphagidae) cultures.
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Analysis of the allergen expression of Blomia tropicalis and Blomia kulagini (Astigmata: Glycyphagidae) cultures.

机译:分析热带浮游生物和Blomia kulagini(Astigmata:Glycyphagidae)培养物的过敏原表达。

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摘要

Laboratory cultures of the mites Blomia tropicalis and Blomia kulagini were used to study the population dynamics of the mites and the kinetics of released allergens during the growth cycle. The analysis of extracts obtained after different incubation periods, by means of immunoblotting, and quantification of the major allergen Blo t 5, allowed definition of three different growth phases, demonstrating that mite cultures during the maximum growth (end of exponential growth curve-beginning maximum growth plateau) contain the largest amount of allergenic components as well as the highest Blo t 5 concentration.
机译:利用螨虫的实验室培养物对热带螨虫和库拉米虫进行了研究,以研究螨虫的种群动态以及生长周期中释放的过敏原的动力学。通过免疫印迹分析不同潜伏期后获得的提取物,并对主要过敏原Blo t 5进行定量分析,从而可以定义三个不同的生长阶段,这表明螨虫培养物在最大生长期间(指数生长曲线的末端开始最大)增长平台)包含最多的变应原成分以及最高的Blo t 5浓度。

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