首页> 外文期刊>Journal of Medical Entomology >Phylogenetic characterization of Wolbachia symbionts infecting Cimex lectularius L. and Oeciacus vicarius Horvath (Hemiptera: Cimicidae).
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Phylogenetic characterization of Wolbachia symbionts infecting Cimex lectularius L. and Oeciacus vicarius Horvath (Hemiptera: Cimicidae).

机译:感染Cimex lectularius L.和Oeciacus副Horvath的Wolbachia共生体的系统发生学特征(双翅目:Cimicidae)。

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摘要

Wolbachia symbionts are obligate intracellular bacteria that cause host reproductive alterations in many arthropods and filarial nematodes. We identified Wolbachia symbionts in the cliff swallow bug (Oeciacus vicarius) and the human bed bug (Cimex lectularius) (Hemiptera: Cimicidae) by polymerase chain reaction (PCR) amplification and sequencing using Wolbachia-specific 16S rDNA and FtsZ primers. Phylogenetic analyses using Bayesian, maximum likelihood, and maximum parsimony algorithms indicated, with strong support, that (1) Wolbachia infections in these two cimicid hosts form a monophyletic group, and (2) the Wolbachia strains detected belong to the F clade, previously associated with termites, weevils, and filarial nematodes.
机译:Wolbachia共生菌是专性的细胞内细菌,可导致许多节肢动物和丝状线虫的宿主繁殖变化。我们通过聚合酶链反应(PCR)扩增和测序,使用Wolbachia特异的16S rDNA和FtsZ引物鉴定了悬崖燕子(Oeciacus vicarius)和人类臭虫(Cimex lectularius)(Hemiptera:Cimicidae)中的Wolbachia共生体。使用贝叶斯,最大似然和最大简约算法的系统发育分析表明,在强有力的支持下,(1)这两个杀螨剂宿主中的Wolbachia感染形成一个单系群,(2)检测到的Wolbachia菌株属于F进化枝,以前与白蚁,象鼻虫和丝状线虫。

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