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首页> 外文期刊>Journal of Medical Entomology >Field validation of Aedes aegypti (Diptera : Culicidae) age estimation by analysis of cuticular hydrocarbons
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Field validation of Aedes aegypti (Diptera : Culicidae) age estimation by analysis of cuticular hydrocarbons

机译：通过分析表皮碳氢化合物对埃及伊蚊（双翅目：葫芦科）年龄估计的现场验证

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In previous studies, we developed linear regression models to age-grade female Aedes aegypti L. reared and maintained under controlled laboratory conditions. The models were based on temporal differences between two cuticular hydrocarbons, pentacosane (C25H52) and nonacosane (C29H60), which were extracted from Ae. aeglipti legs and analyzed by gas-liquid chromatography. These initial models predicted adult female age rip to 165 DD (12-15 calendar d at 28degreesC). The age of older mosquitoes, however, could not be accurately predicted. In this study, our original regression models were tested using age data obtained from mosquitoes maintained in afield laboratory and those that were marked, released, and recaptured in northwestern Thailand. Our field data led to the development of two new regression models: one for the cool-dry season (February-March) and one for the rainy season (July-August). Both models resulted in better estimates of age than the original model and thus improved our ability to predict the age of Ae. aegypti to 15 calendar d. Females older than 15 d can be identified as such, but their exact age cannot yet be estimated. The new models will be useful for epidemiological studies and evaluating the impact of Ae. aeglipti control interventions for disease prevention.
机译：在以前的研究中，我们开发了线性回归模型，以对在受控实验室条件下饲养和维持的雌性埃及伊蚊进行了分类。该模型基于从Ae提取的两种表皮碳氢化合物（五碳烷（C25H52）和壬二烷（C29H60））的时间差异。 aeglipti腿，并通过气液色谱法进行分析。这些初始模型预测成年女性的年龄会下降到165 DD（在28℃下为12-15个日历日）。但是，无法准确预测年龄较大的蚊子的年龄。在这项研究中，我们使用从现场实验室中保存的蚊子获得的年龄数据以及在泰国西北部被标记，释放和重新捕获的蚊子的年龄数据测试了我们的原始回归模型。我们的现场数据导致开发了两种新的回归模型：一种用于凉爽干燥季节（2月至3月），另一种用于雨季（7月至8月）。与原始模型相比，这两种模型都能更好地估计年龄，从而提高了我们预测Ae年龄的能力。埃及至15个日历d。可以确定年龄大于15 d的女性，但尚不能估计其确切年龄。新模型将对流行病学研究和评估Ae的影响有用。预防疾病的aeglipti控制干预措施。

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