Identification of four vectors of human Plasmodium spp. by multiplex PCR: Anopheles rangeli, An. strodei, An. triannulatus, and An. trinkae (Diptera: Culicidae: Nyssorhynchus).

Fritz G N; Engman S; Rodriguez R; Wilkerson R C

首页> 外文期刊>Journal of Medical Entomology >Identification of four vectors of human Plasmodium spp. by multiplex PCR: Anopheles rangeli, An. strodei, An. triannulatus, and An. trinkae (Diptera: Culicidae: Nyssorhynchus).

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Identification of four vectors of human Plasmodium spp. by multiplex PCR: Anopheles rangeli, An. strodei, An. triannulatus, and An. trinkae (Diptera: Culicidae: Nyssorhynchus).

机译：鉴定人疟原虫属物种的四个载体。通过多重PCR：按蚊（An按蚊）。 Strodei，安triannulatus和安Trinkae（双翅目：Cu科：Nyssorhynchus）。

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One of the major obstacles for studies of the biology, ecology, and behaviour of Neotropical vectors of human Plasmodium has been the lack of reliable and efficient means of identifying many species. Although the subgenus Nyssorhynchus includes most species responsible for human transmission in South America, there are no polymerase chain reaction (PCR)-based techniques for identifying members of this taxon. We describe the first multiplex PCR for identifying four species in the subgenus Nyssorhynchus that are vectors of human Plasmodium spp. Four species specific primers, together with a universal primer that anneals to the 5.8S rDNA region, produce amplicons of the internal transcribed spacer two with base pair sizes of 131, 308, 371, and 441 for An. triannulatus, An. trinkae, An. strodei, and An. rangeli, respectively.

机译：研究人类疟原虫新嗜性载体的生物学，生态学和行为的主要障碍之一是缺乏识别多种物种的可靠而有效的手段。尽管Nyssorhynchus属包括南美洲负责人类传播的大多数物种，但尚没有基于聚合酶链反应（PCR）的技术来识别该分类单元的成员。我们描述了第一个多重PCR，用于鉴定Nyssorhynchus亚属中的四个物种，它们是人类疟原虫属物种的载体。四种特定引物以及与5.8S rDNA区域退火的通用引物一起，产生内部转录间隔区的扩增子，两个间隔区的碱基对大小分别为131、308、371和441。三角藻Trinkae，安strodei和An。兰格里。

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《Journal of Medical Entomology》 |2004年第6期|共5页
作者
Fritz G N; Engman S; Rodriguez R; Wilkerson R C;
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正文语种 eng
中图分类病理学;
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disease vectors; human diseases; malaria; mosquito borne diseases; polymerase chain reaction; protozoal infections; ribosomal DNA;

机译：疾病载体;人类疾病;疟疾;蚊媒疾病;聚合酶链反应;原生动物感染;核糖体DNA;

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1. Identification of four vectors of human Plasmodium spp. by multiplex PCR: Anopheles rangeli, An. strodei, An. triannulatus, and An. trinkae (Diptera: Culicidae: Nyssorhynchus). [J] . Fritz G N, Engman S, Rodriguez R, Journal of Medical Entomology . 2004,第6期

机译：鉴定人疟原虫属物种的四个载体。通过多重PCR：按蚊（An按蚊）。 Strodei，安triannulatus和安Trinkae（双翅目：Cu科：Nyssorhynchus）。
2. Incrimination of Anopheles (Anopheles) intermedius Peryassú, An. (Nyssorhynchus) nuneztovari Gabaldón, An. (Nys.) oswaldoi Peryassú as natural vectors of Plasmodium falciparum in French Guiana [J] . Isabelle Dusfour, Jean Issaly, Pascal Gaborit, Memórias do Instituto Oswaldo Cruz . 2012,第3期

机译：中间人按蚊（Anopheles）interdyiusPeryassú的罪名。（Nyssorhynchus）nuneztovariGabaldón，An。（Nys。）oswaldoiPeryassú是法属圭亚那恶性疟原虫的天然载体
3. Incrimination of Anopheles Anopheles) intermedius Peryassú, An. ( Nyssorhynchus) nuneztovari Gabaldón, An. ( Nys.) oswaldoi Peryassú as natural vectors of Plasmodium falciparum in French Guiana [J] . Dusfour Isabelle, Issaly Jean, Carinci Romuald, Memorias do Instituto Oswaldo Cruz . 2012,第3期

机译：按蚊的罪名（Anopheles）intermediusPeryassú，An。（Nyssorhynchus）nuneztovariGabaldón，An。（Nys。）oswaldoiPeryassú是法属圭亚那恶性疟原虫的天然载体
4. Contribution of Anopheles funestus An. gambiae and An. nili (Diptera: Culicidae) to the perennial malaria transmission in the southern and western forest areas of Côte d’Ivoire [O] . A M Adja, E K N’goran, B G Koudou, 2011

机译：Anesteles funestusAn。的贡献。冈比亚和安。 nili（Diptera：Culicidae）与科特迪瓦南部和西部森林地区的常年疟疾传播
5. Incrimination of Anopheles (Anopheles) intermedius Peryassú, An. (Nyssorhynchus) nuneztovari Gabaldón, An. (Nys.) oswaldoi Peryassú as natural vectors of Plasmodium falciparum in French Guiana [O] . Isabelle Dusfour, Jean Issaly, Romuald Carinci, 2012

机译：中间人按蚊（Anopheles intermediusPeryassú，An。（Nyssorhynchus）nuneztovariGabaldón，An。（Nys。）oswaldoiPeryassú是法属圭亚那恶性疟原虫的天然载体
6. Synonymy of Anopheles (Nyssorhynchus) noroestensis with An. (Nys.) evansi, with a Description of the Male Genitalia of the Lectotype of An. (Nys.) evansi (Diptera: Culicidae) [R] . Faran, M. E. 1981

机译：anopheles（Nyssorhynchus）noroestensis与an的同义词。（Nys。）evansi，描述了an的Lectotype的男性生殖器。（Nys。）evansi（双翅目：蚊科）

Identification of four vectors of human Plasmodium spp. by multiplex PCR: Anopheles rangeli, An. strodei, An. triannulatus, and An. trinkae (Diptera: Culicidae: Nyssorhynchus).

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