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首页> 外文期刊>Journal of Medical Entomology >Inheritance and mechanism of resistance to Bacillus sphaericus in Culex quinquefasciatus (Diptera : Culicidae) from China and Brazil
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Inheritance and mechanism of resistance to Bacillus sphaericus in Culex quinquefasciatus (Diptera : Culicidae) from China and Brazil

机译：中国和巴西的库克斯库蚊（Dilexera：Culicidae）对球形芽孢杆菌的遗传及抗性机制

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摘要

Investigations on the inheritance and mechanism of resistance to Bacillus sphaericus Neide in Culex quinquefasciatus Say colonies, selected with strains C3-41 (RLCq1/C3-41) and 2362 (CqRL1/2362), were performed in China and Brazil, respectively. The progeny of reciprocal F1 crosses (susceptible female X resistant male and vice versa) from both resistant colonies responded alike in bioassays, indicating recessive inheritance. Data on larvae susceptibility from the backcross offspring between F1 and their respective susceptible and resistant parental colonies are consistent with a monofactorial and autosomal mode of inheritance. In vitro binding assays between 125 1 binary (Bin2) toxin and the brush border membrane fractions (BBMF) from CqRL1/2362 and RLCq1/C3-41 larvae showed that resistance, in both colonies, is caused by a failure in the binding step of the B. sphaericus Bin2 toxin to its specific midgut receptor. The specific and saturable binding of Bin2 toxin to BBMF from F1 larvae (CqRL1/2362 X susceptible counterpart) confirms the recessive inheritance of the resistance gene. Further studies are needed to advance understanding of B. sphaericus resistance.
机译：在中国和巴西分别进行了由C3-41（RLCq1 / C3-41）和2362（CqRL1 / 2362）菌株选择的库克斯库克斯（Culex quinquefasciatus Say）菌落对球形芽孢杆菌内德氏菌的遗传和抗性机制的研究。在生物测定中，来自两个抗性菌落的反向F1杂交的子代（易感的雌性X抗性雄性，反之亦然）的反应相似，表明是隐性遗传。 F1与它们各自的易感和抗性父母群体之间的回交后代的幼虫易感性数据与单因素和常染色体遗传模式一致。在125 1二元（Bin2）毒素与CqRL1 / 2362和RLCq1 / C3-41幼虫的刷状缘膜级分（BBMF）之间的体外结合测定表明，在两个菌落中，抗性是由于结合步骤失败导致的球形芽孢杆菌Bin2毒素对其特定的中肠受体起作用。 Bin2毒素与F1幼虫的BBMF的特异性和饱和结合（CqRL1 / 2362 X易感对应物）证实了抗性基因的隐性遗传。需要进一步的研究以增进对球形芽孢杆菌抗性的了解。

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