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首页> 外文期刊>Journal of Medical Entomology >A technique for longitudinally sampling individual adult Ixodes scapularis (Acari: Ixodidae)
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A technique for longitudinally sampling individual adult Ixodes scapularis (Acari: Ixodidae)

机译：纵向对单个成年I肩I虫（Acari：Ixodidae）进行采样的技术

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摘要

The first technique for repeatedly sampling individual Ixodes scapularis adult ticks was developed and validated. Gut samples from the same individual ticks were removed and analyzed at weekly intervals. Multiple analyses were conducted on each gut sample (e.g., total protein concentration, presence of viable B. burgdorferi spirochetes, and concentration of outer surface protein A [OspA]). Female I. scapularis survived for up to 25 d after gut sampling. Seventy-five percent of females oviposited after the sampling procedure, with 14% of ticks laying >1,500 eggs. No significant differences in either fecundity or length of survival were found between B. burgdorferi-infected and uninfected I. scapularis. This technique will enable longitudinal studies on both tick-pathogen interactions and physiological studies that have hitherto not been attempted.
机译：开发并验证了第一种用于反复采样肩repeatedly突舌成年I的技术。取下来自相同个体tick的肠样本，并每周进行一次分析。对每个肠道样品进行了多次分析（例如，总蛋白浓度，活的B. burgdorferi螺旋菌的存在以及外表面蛋白A [OspA]的浓度）。肠道采样后，雌性肩cap鱼存活长达25 d。抽样程序后有75％的雌性产卵，其中14％的s虫产下了1,500多个卵。在感染伯氏疏螺旋体和未感染肩骨之间，在繁殖力或存活时间上均无显着差异。这项技术将使对tick-病原体相互作用的纵向研究和迄今为止尚未尝试的生理学研究成为可能。

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