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首页> 外文期刊>Journal of Medical Entomology >Genomic Sequence and Phylogenetic Analysis of Culex Flavivirus, an Insect-Specific Flavivirus, Isolated From Culex pipiens (Diptera: Culicidae) in Iowa
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Genomic Sequence and Phylogenetic Analysis of Culex Flavivirus, an Insect-Specific Flavivirus, Isolated From Culex pipiens (Diptera: Culicidae) in Iowa

机译：从爱荷华州的淡色库蚊（Dilexera：Culicidae）分离的昆虫特异性黄病毒库蚊黄病毒的基因组序列和系统发育分析。

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Adult mosquitoes (Diptera: Culicidae) were collected in 2007 and tested for specific viruses, including West Nile virus, as part of the ongoing arbovirus surveillance efforts in the state of Iowa. A subset of these mosquitoes (6,061 individuals in 340 pools) was further tested by reverse transcription-polymerase chain reaction (RT-PCR) using flavivirus universal primers. Of the 211 pools of Cidex pipiens (L.) tested, 50 were positive. One of 51 pools of Culex tarsalis Coquillet was also positive. The flavivirus minimum infection rates (expressed as the number of positive mosquito pools per 1,000 mosquitoes tested) for Cx. pipiens and Cx. tarsalis were 10.3 and 1.2, respectively. Flavivirus RNA was not detected in Aedes triseriatus (Say) (52 pools), Culex erraticus (Dyar & Knab) (25 pools), or Culex territans Walker (one pool). Sequence analysis of all RT-PCR products revealed that the mosquitoes bad been infected with Culex flavivirus (CxFV), an insect-specific virus previously isolated in Japan, Indonesia, Texas, Mexico, Guatemala and Trinidad. The complete genome of one isolate was sequenced, as were the envelope protein genes of eight other isolates. Phylogenetic analysis revealed that CxFV isolates from the United States (Iowa and Texas) are more closely related to CxFV isolates front Asia than those from Mexico, Guatemala, and Trinidad.
机译：作为爱荷华州正在进行的虫媒病毒监测工作的一部分，2007年收集了成年蚊子（双翅目：Culicidae）并测试了特定病毒，包括西尼罗河病毒。使用黄病毒通用引物通过逆转录-聚合酶链反应（RT-PCR）进一步测试了这些蚊子的一个子集（340个库中的6,061个个体）。在测试的211个Pipiens pipiens（L.）库中，有50个呈阳性。库雷克斯库塔库利库尔库的51个库之一也呈阳性。黄病毒对Cx病毒的最低感染率（表示为每千只蚊子的阳性蚊子池数）。 pipiens和Cx。分别为10.3和1.2。在白纹伊蚊（Say）（52个池），库克斯（Culex erraticus）（Dyar＆Knab）（25个池）或库蚊（Culex territans Walker）（一个池）中未检测到黄病毒RNA。对所有RT-PCR产物的序列分析表明，坏死的蚊子感染了库克斯黄病毒（CxFV），这是一种以前在日本，印度尼西亚，德克萨斯州，墨西哥，危地马拉和特立尼达分离的昆虫特异性病毒。对一种分离株的完整基因组进行测序，对其他八种分离株的包膜蛋白基因进行测序。系统发育分析表明，与来自墨西哥，危地马拉和特立尼达的CxFV分离株相比，来自美国（爱荷华州和德克萨斯州）的CxFV分离株与亚洲前部的CxFV分离株关系更密切。

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