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首页> 外文期刊>Journal of Medical Entomology >Detection of Rickettsia rickettsii and Bartonella henselae in Rhipicephalus sanguineus ticks from California
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Detection of Rickettsia rickettsii and Bartonella henselae in Rhipicephalus sanguineus ticks from California

机译:加利福尼亚州的Rhipicephalus sanguineus壁虱中立克次体立克次体和汉氏巴尔通体的检测

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摘要

Sixty-two questing adult Rhipicephalus sanguineus (Latreille) ticks were collected by direct removal from blades of turfgrass and adjacent concrete walkways at a suburban home in Riverside County, CA, and tested for the presence of Rickettsia, Bartonella, and Ehrlichia DNA. Polymerase chain reaction (PCR) was used to amplify fragments of the 17-kDa antigen gene and the rOmpA gene of the spotted fever group rickettsiae. One male tick contained R. rickettsii DNA; its genotype differed from R. rickettsii isolates found in Montana and Arizona that cause Rocky Mountain spotted fever and from Hlp#2 and 364D serotypes. One male tick and one female tick contained B. henselae DNA. No Ehrlichia platys or Ehrlichia canis DNAs were detected using nested PCR for their 16S rRNA genes. These findings extend the area where Rickettsia rickettsii may be vectored by Rh. sanguineus. Rh. sanguineus also may be infected with Bartonella henselae, a human pathogen that is typically associated with fleas and causes cat scratch disease.
机译:在加利福尼亚州里弗赛德县的一个郊区住宅中,通过直接从草坪草和邻近的混凝土走道的叶片上除去,收集了62只成年Rhipicephalus sanguineus(Latreille)的tick,并测试了立克次体,Bartonella和Ehrlichia DNA的存在。聚合酶链反应(PCR)用于扩增点状发热立克次体的17-kDa抗原基因和rOmpA基因的片段。一个雄性壁虱包含里氏立克次体DNA;它的基因型不同于在蒙大拿州和亚利桑那州发现的导致落基山斑疹热的立克次氏菌分离株以及Hlp#2和364D血清型。一只雄性tick和一个雌性tick包含亨氏芽孢杆菌DNA。使用巢式PCR对其16S rRNA基因未检测到埃里希板栗或犬埃里希氏菌DNA。这些发现扩大了立克次体可能由Rh介导的立克次体的区域。 sanguineus。铑sanguineus也可能感染了汉通巴尔通体(Bartonella henselae),后者是一种通常与跳蚤相关并引起猫抓挠性疾病的人类病原体。

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