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首页> 外文期刊>Journal of Medical Entomology >Host antibodies in mosquito bloodmeals: a potential tool to detect and monitor infectious diseases in wildlife.
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Host antibodies in mosquito bloodmeals: a potential tool to detect and monitor infectious diseases in wildlife.

机译：蚊子血粉中的宿主抗体：一种检测和监测野生生物中传染病的潜在工具。

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When a female mosquito bites, it carries away a blood sample containing specific antibodies that can provide a history of the immune responses of its vertebrate host. This research examines the limits and reliability of a technique to detect antibodies in blood-fed mosquitoes in the laboratory. Mosquitoes were fed on blood containing a specific antibody, and then they were assayed using an enzyme-linked immunosorbent assay to determine the limits of detection of antibody over time, at different temperatures and initial antibody concentrations. The antibody, at an initial concentration of 1 microg/ml, could be detected in mosquitoes for 24-48 h after feeding. Blind tests simulating the assay of feral mosquitoes were used to test the reliability of the method and detected positive mosquitoes with few false negatives and no false positives. Specific antibodies also could be detected in mosquitoes that had been air-dried or preserved in ethanol. This research indicates that, in theory, the collection and immunological assay of blood-fed mosquitoes could be developed to detect and monitor infectious disease in wildlife.
机译：当雌性蚊子叮咬时，它会带走包含特定抗体的血液样本，这些抗体可以提供其脊椎动物宿主免疫反应的历史。这项研究检验了在实验室中检测血液喂养的蚊子中抗体的技术的局限性和可靠性。将蚊子喂入含有特定抗体的血液中，然后使用酶联免疫吸附法对其进行分析，以确定在不同温度和初始抗体浓度下随时间推移检测抗体的极限。喂食后24-48小时内可在蚊子中检测到初始浓度为1微克/毫升的抗体。模拟野性蚊子试验的盲法测试用于测试方法的可靠性，并检测出假阴性少，假阳性少的阳性蚊子。也可以在风干或保存在乙醇中的蚊子中检测到特异性抗体。这项研究表明，从理论上讲，可以开发采血蚊子的收集和免疫学检测方法，以检测和监测野生动植物的传染病。

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