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首页> 外文期刊>Journal of Medical Entomology >Ticks (Acari: Ixodidae) Infesting Wild Birds in an Atlantic Forest Area in the State of ScDo Paulo, Brazil, with Isolation of Rickettsia from the Tick Amblyomma longirostre
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Ticks (Acari: Ixodidae) Infesting Wild Birds in an Atlantic Forest Area in the State of ScDo Paulo, Brazil, with Isolation of Rickettsia from the Tick Amblyomma longirostre

机译：壁虱（Acari：Ixodidae）在巴西ScDo Paulo州的大西洋森林地区感染野生鸟类，并从壁虱Aicklyomma longirostre中分离出立克次体

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摘要

During field work in Nazarcb Paulista, state of ScDo Paulo, Brazil, we found 13 (56.5%) of 23 birds (mostly Passeriformes) to be infested by 28 larvae and 1 nymph of Amblyomma spp. Two larvae were reared to the adult stage, being taxonomically identified as Amblyomma parkeri Fonseca and AragcDo, whereas five larvae and one nymph were identified as Amblyomma longirostre Koch. All six A. longirostre specimens were shown to be infected by rickettsia, as demonstrated by polymerase chain reaction (PCR) targeting two rickettsial genes (gltA and ompA) or isolation of rickettsia in cell culture from one of the ticks. This isolate was designated as strain AL, which was established in Vero cell culture and was molecularly characterized by DNA sequencing fragments of the rickettsial genes gltA, htrA, ompA, and ompB. Phylogenetic analyses inferred from ompA and ompB partial sequences showed a high degree of similarity of strain AL with Rickettsia sp. strain ARANHA, previously detected by PCR in A. longirostre ticks from Rondctnia, northern Brazil. We conclude that strain AL is a new rickettsia genotype belonging to the same species of strain ARANHA, which are closely related to Candidatus 'R. amblyommii'. Further studies should elucidate if strains AL and ARANHA are different strains of Candidatus 'R. amblyommii' or are a new species.
机译：在巴西ScDo Paulo州的Nazarcb Paulista进行的实地调查中，我们发现23羽鸟类（主要是Passeriformes）中有13羽（56.5％）被28只幼虫和1只若虫Amblyomma spp侵染。从分类学上将两个幼虫饲养到成年阶段，在分类学上被鉴定为Ambclyomma parkeri Fonseca和AragcDo，而将五个幼虫和一个若虫鉴定为Amblyomma longirostre Koch。通过靶向两个立克次体基因（gltA和ompA）的聚合酶链反应（PCR）或从壁虱中分离出立克次体，证明了所有六个长曲霉标本都感染了立克次体。该分离株被命名为AL株，其在Vero细胞培养物中建立，并通过立克次体基因gltA，htrA，ompA和ompB的DNA测序片段进行分子表征。从ompA和ompB部分序列推断的系统发育分析显示AL菌株与立克次体sp。高度相似。菌株ARANHA，先前已通过PCR在来自巴西北部Rondctnia的A. longirostre s中检测到。我们得出的结论是，AL菌株是属于立克次体的新基因型，属于ARANHA菌株的同一物种，与Candidatus'R密切相关。 amblyommii'。进一步的研究应阐明菌株AL和ARANHA是否为Candidatus'R的不同菌株。 amblyommii'或是一个新物种。

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