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首页> 外文期刊>Journal of Medical Entomology >Persistence of Buggy Creek Virus (Togaviridae, Alphavirus) for Two Years in Unfed Swallow Bugs (Hemiptera: Cimicidae: Oeciacus vicarius)
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Persistence of Buggy Creek Virus (Togaviridae, Alphavirus) for Two Years in Unfed Swallow Bugs (Hemiptera: Cimicidae: Oeciacus vicarius)

机译：Buggy Creek病毒（Togaviridae，Alphavirus）在未喂食的燕子虫（半翅目：Cimicidae：Oeciacus vicarius）中持续存在两年。

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Alphaviruses (Togaviridae) have rarely been found to persist for long in the adult insects that serve as their vectors. The ectoparasitic swallow bug (Hemiptera: Cimicidae: Oeciacus vicarius Horvath), the vector for Buggy Creek virus (BCRV; Togaviridae, Alphavirus), lives year-round in the mud nests of its host, the cliff swallow (Petrochelidon pyrrhonota Vieillot). We measured the prevalence of BCRV in swallow bugs at sites with cliff swallows present and at the same sites after cliff swallows had been absent for 2 yr. We collected bugs directly from cliff swallow nests in the field and screened bug pools with BCRV-specific real-time-polymerase chain reaction (RT-PCR) and plaque assay. At two colony sites last occupied by birds 2 yr earlier, we found 12.5 and 55.6% of bug pools positive for BCRV RNA by RT-PCR. Infection rates (per 1,000 bugs) for these sites were 1.32 and 7.39. RNA prevalence in the unfed bugs was not significantly different from that in fed bugs 2 yr earlier at the same sites. The RNA-positive samples from unfed bugs failed to yield cytopathic BCRV by Vero-cell plaque assay. However, viral RNA concentrations did not differ between unfed bugs and bugs at active sites, and over 84% of positive bug pools were cytopathic to Vero cells 4-5 wk later, after cliff swallows moved into one of the colony sites. These data demonstrate the persistence of potentially infectious BCRV in unfed swallow bugs for at least 2 yr in nature.
机译：很少发现甲病毒（Togaviridae）在作为其载体的成年昆虫中能够长期存在。 Buggy Creek病毒的载体（BCRV; Togaviridae，Alphavirus）的寄生寄生虫（半翅目：Cimicidae：Oeciacus vicarius Horvath）整年生活在其寄主的泥巢燕子（Petrochelidon pyrrhonota Vieillot）中。我们测量了存在燕子的地点和没有燕子2年后的同一地点燕子中的BCRV的患病率。我们直接从野外的燕子燕窝收集臭虫，并使用BCRV特异性实时聚合酶链反应（RT-PCR）和噬菌斑分析筛选了臭虫库。在2年之前最后被鸟占领的两个殖民地站点，我们通过RT-PCR发现BCRV RNA阳性的虫子库的12.5和55.6％。这些网站的感染率（每1000个错误）为1.32和7.39。未饲喂小虫中的RNA流行率与2年前早些时候在同一地点的饲喂小虫中的RNA流行没有显着差异。未喂食的虫子的RNA阳性样品无法通过Vero细胞噬菌斑测定产生细胞病性BCRV。但是，未喂食的臭虫和活动位点的臭虫之间的病毒RNA浓度没有差异，并且在超过4-5周后，悬崖燕子移入其中一个菌落位点后，超过84％的阳性臭虫库对Vero细胞具有细胞毒性。这些数据表明，自然界中至少2年内，未进食的燕子中可能具有传染性的BCRV持续存在。

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