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首页> 外文期刊>Journal of Medical Entomology >Prevalence of Rickettsia felis and the First Identification of Bartonella henselae Fizz/CAL-1 in Cat Fleas (Siphonaptera: Pulicidae) from Taiwan
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Prevalence of Rickettsia felis and the First Identification of Bartonella henselae Fizz/CAL-1 in Cat Fleas (Siphonaptera: Pulicidae) from Taiwan

机译：来自台湾的猫蚤（Siphonaptera：Pulicidae）中立克次体的患病率和亨氏巴尔通体Fizz / CAL-1的首次鉴定

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Cat fleas (Ctenocephalides felis [Bouchee]) are the primary ectoparasites of dog and cat populations. In this study, we report the monthly population dynamics of Rickettsia felis and Bartonella spp. (two zoonotic pathogens that can cause human disease) in cat fleas collected from dogs and cats in Taipei, Taiwan, from December 2006 to December 2007. Natural R. felis infection in individual cat fleas was assessed by polymerase chain reaction (PCR) using pRF-, ompB-, and gltA-specific primer pairs. Samples positive by PCR were confirmed with DNA sequencing. R. felis was detected in cat fleas year round, and the average infection rate was 21.4% (90 of 420) in 2007. Cat fleas also play an important role in the transmission of Bartonella between reservoirs and other mammalian hosts. In this study, we used primer pairs specific for the Bartonella gltA and rpoB genes to detect Bartonella infections. Of the 420 cat fleas tested, 38 were positive by PCR for Bartonella. Sequence similarities to Bartonella henselae, Bartonella clarridgeiae, and Bartonella koehlerae were observed in 6.2% (26 of 420), 2.1% (9 of 420), and 0.7% (3 of 420) of the fleas, respectively. Based on the pap31 gene sequence, several amplicons of the B. henselae detected in the cat fleas could be subgrouped into three strains: Fizz/CAL-1 (n = 18), Marseille (n = 5), and Houston-1 (n = 3). These results demonstrate that cat fleas infected with R. felis are endemic to Taiwan, and highlight the role of C. felis in Bartonella transmission between reservoirs and other mammal hosts and demonstrate the genetic variability of B. henselae in Taiwan.
机译：猫蚤（Ctenocephalides felis [Bouchee]）是狗和猫种群的主要外寄生物。在这项研究中，我们报告了立克次体和巴尔通体的每月种群动态。 2006年12月至2007年12月，从台湾台北的猫狗身上采集的猫蚤中的两种人畜共患病病原体（可能导致人类疾病的病原体）。使用pRF通过聚合酶链反应（PCR）评估了个别猫蚤中的自然R. felis感染-，ompB-和gltA特异性引物对。 PCR阳性的样品用DNA测序确认。全年在猫蚤中检出了R. felis，2007年的平均感染率为21.4％（420个中的90个）。猫蚤在巴尔通体在水库与其他哺乳动物宿主之间的传播中也起着重要作用。在这项研究中，我们使用了针对Bartonella gltA和rpoB基因的引物对来检测Bartonella感染。通过PCR检测，在420只猫蚤中，巴尔通体呈阳性。分别在跳蚤中的6.2％（420个中的26个），2.1％（420个中的9个）和0.7％（420个中的3个）中观察到与汉氏巴尔通体，克拉氏巴尔通体和科氏巴尔通体的序列相似性。根据pap31基因序列，在猫蚤中检测到的汉逊芽孢杆菌的几个扩增子可分为三个菌株：Fizz / CAL-1（n = 18），Marseille（n = 5）和Houston-1（n = 3）。这些结果表明，感染了猫科动物的猫蚤是台湾特有的，并突出了猫科动物在巴尔通体在水库与其他哺乳动物寄主之间传播的作用，并证明了台湾汉魏杆菌的遗传变异性。

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