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首页> 外文期刊>Journal of Medical Entomology >An insight into the microbiome of the Amblyomma maculatum (Acari: Ixodidae).
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An insight into the microbiome of the Amblyomma maculatum (Acari: Ixodidae).

机译：深入了解斑盲A（Acari：Ixodidae）的微生物组。

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The aim of this study was to survey the bacterial diversity of Amblyomma maculatum Koch, 1844, and characterize its infection with Rickettsia parkeri. Pyrosequencing of the bacterial 16S rRNA was used to determine the total bacterial population in A. maculatum. Pyrosequencing analysis identified Rickettsia in A. maculatum midguts, salivary glands, and saliva, which indicates successful trafficking in the arthropod vector. The identity of Rickettsia spp. was determined based on sequencing the rickettsial outer membrane protein A (rompA) gene. The sequence homology search revealed the presence of R. parkeri, Rickettsia amblyommii, and Rickettsia endosymbiont of A. maculatum in midgut tissues, whereas the only rickettsia detected in salivary glands was R. parkeri, suggesting it is unique in its ability to migrate from midgut to salivary glands, and colonize this tissue before dissemination to the host. Owing to its importance as an emerging infectious disease, the R. parkeri pathogen burden was quantified by a rompB-based quantitative polymerase chain reaction (qPCR) assay and the diagnostic effectiveness of using R. parkeri polyclonal antibodies in tick tissues was tested. Together, these data indicate that field-collected A. maculatum had a R. parkeri infection rate of 12-32%. This study provides an insight into the A. maculatum microbiome and confirms the presence of R. parkeri, which will serve as the basis for future tick and microbiome interaction studies.
机译：这项研究的目的是调查1844年斑斑苹果草（Amblyomma maculatum Koch）的细菌多样性，并表征其被立克次体立克次体感染。细菌16S rRNA的焦磷酸测序用于确定黄斑曲霉中的总细菌种群。焦磷酸测序分析确定了斑纹拟南芥中肠，唾液腺和唾液中的立克次体，这表明节肢动物媒介已成功贩运。立克次体的身份。基于对立克次体外膜蛋白A（rompA）基因的测序确定。序列同源性搜索显示在中肠组织中存在黄斑曲霉R.parkeri，立克次氏菌和内生立克次体，而唾液腺中唯一检测到的立克次体是R.parkeri，这表明它具有从中肠迁移的独特能力。唾液腺，并在传播到宿主之前定居此组织。由于其作为新兴传染病的重要性，通过基于rompB的定量聚合酶链反应（qPCR）测定法定量了帕克氏菌的病原体负担，并测试了在壁虱组织中使用帕克氏菌多克隆抗体的诊断效力。总之，这些数据表明田间采集的黄斑曲霉的帕克氏杆菌感染率为12-32％。这项研究提供了对A. maculatum微生物组的见解，并证实了R. parkeri的存在，这将成为未来tick和微生物组相互作用研究的基础。

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