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首页> 外文期刊>Journal of Medical Entomology >Prevalence of Ehrlichia chaffeensis (Rickettsiales: Rickettsiaeae) in Amblyomma americanum (Acari: Ixodidae)From the Georgia coast and barrier islands
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Prevalence of Ehrlichia chaffeensis (Rickettsiales: Rickettsiaeae) in Amblyomma americanum (Acari: Ixodidae)From the Georgia coast and barrier islands

机译：佐治亚州沿岸和障碍岛上的美洲Amblyomma（Acari：Ixodidae）中的恰菲埃里希氏菌（Rickettsiales：Rickettsiaeae）患病率

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摘要

ABSTRACT Human monocytic ehrliehiosis is an emerging zoonosis caused by infection with Ehrlichia chaffeensis Anderson, Dawson, Jones & Wilson. which is transmitted to mammals by ixodid ticks. Prevalence of infected ticks and distribution of infection foci indicate relative risk of human exposure to ehrlichiosis and may be influenced by factors such as geographic isolation, human disturbance, and the availability of suitable mammalian reservoir hosts. To test this, individual and pooled lone star ticks, Amblyomma americanum (L.), were collected from three populations from the Georgia coast (1 mainland site and two barrier islands) and screened for E. chaffeensis. A species-specific, nested polymerase chain reaction (PCR) assay was used to amplify a 572 bp fragment of the E. chaffeensis 16S rRNA gene from infected ticks. PCR product specificity was confirmed b x nucleotide sequencing. Our results showed the prevalence of infected ticks to be 0.0% (n = 151), 0.9% (n = 111), and 9.3% (n = 129) for Sapelo Island, St. Catherine's Island, and Fort McAllister, respectively.
机译：摘要人单核细胞增多症是一种新兴的人畜共患病，它是由恰菲埃里希氏体感染引起的。安德森，道森，琼斯和威尔逊。通过虫传播到哺乳动物。感染tick的流行和感染病灶的分布表明人类暴露于埃希氏菌病的相对风险，并且可能受到诸如地理隔离，人类干扰以及合适的哺乳动物宿主来源等因素的影响。为了对此进行测试，从佐治亚州海岸的三个种群（1个内陆站点和两个隔离岛）中收集了单个和集合的孤星tick，美洲美洲mb（L。），并筛选了恰菲埃里希氏菌。使用物种特异性的巢式聚合酶链反应（PCR）分析从受感染的s中扩增出恰菲大肠杆菌16S rRNA基因的572 bp片段。通过核苷酸测序证实了PCR产物的特异性。我们的结果表明，萨佩洛岛，圣凯瑟琳岛和麦克阿里斯特堡的tick虫感染率分别为0.0％（n = 151），0.9％（n = 111）和9.3％（n = 129）。

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