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首页> 外文期刊>Journal of Medical Entomology >Resistance to Bacillus sphaericus in Culex pipiens (Diptera : Culicidae)interaction between recessive mutants and evolution in southern France
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Resistance to Bacillus sphaericus in Culex pipiens (Diptera : Culicidae)interaction between recessive mutants and evolution in southern France

机译:法国南部隐性突变体对淡色库蚊(Cuptidae)的球形芽孢杆菌的抗性

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In southern France, failure to control Culex pipiens L. with Bacillus sphaericus Neide toxin (Bs) was first detected in 1994, at the extreme east of the Languedoc-Roussillon coast. This failure was due to a single recessive mutant, sp-1(R). Two complementary strategies were used to test whether sp-1(R) had invaded the Bs-controlled area by 1998. First, a strain (BP) was selected from resistant larvae sampled in the western part of the Bs-controlled area. In BP strain, resistance involved a single recessive gene, sp-2(R), distinct from sp-1(R), that conferred a similarly high resistance in the homozygous state (approximate to6,000-fold). Combining one copy of sp-1(R) and one of sp-2(R) conferred a > 100-fold resistance. Second, Bs-resistance was monitored among the offspring of field females crossed to sp-1(RR) homozygous males. Females were sampled in 20 localities of southern France and three localities of the Llobregat delta (Barcelona, Spain) where C. pipiens control is also intensive. The 537 females in the study produced enough larvae to infer their genotype: 462 progenies were susceptible and the survival rate of 51 others was explained by the presence of sp-1(R) and/or sp-2(R). The remaining 24 cases indicated that other factors could confer resistance when combined with sp-1(R). The current data showed that, even when recessive, resistant mutants can rapidly increase in frequency, providing some interactions that protect them from disappearance. We discuss the consequences of this finding on the current strategies aimed to avoid or delay resistance in the pests controlled with B. sphaericus or B. thuringiensis Berliner toxins.
机译:在法国南部,1994年首次在朗格多克-鲁西永(Languedoc-Roussillon)海岸的最东端检测到未能用球形芽孢杆菌Neide毒素(Bs)控制淡色库蚊。该失败是由于单个隐性突变体sp-1(R)引起的。两种互补策略用于测试sp-1(R)到1998年是否已入侵Bs控制区。首先,从在Bs控制区西部采样的抗性幼虫中选择一种菌株(BP)。在BP菌株中,抗药性涉及与sp-1(R)不同的单个隐性基因sp-2(R),在纯合状态下赋予相似的高抗性(约6000倍)。将一个拷贝的sp-1(R)和一个拷贝的sp-2(R)结合可产生> 100倍的抗性。其次,在与sp-1(RR)纯合雄性杂交的田间雌性后代中监测了Bs抗性。在法国南部的20个地区和Llobregat三角洲的三个地区(西班牙的巴塞罗那)进行了雌性采样,对pipiens的控制也很严格。该研究中的537名雌性产生了足够的幼虫以推断其基因型:易感的462个子代,sp-1(R)和/或sp-2(R)的存在解释了其他51个子代的存活率。其余24例表明与sp-1(R)结合使用时,其他因素也可能产生抗药性。目前的数据表明,即使是隐性抗性突变体,其频率也可以迅速增加,从而提供了一些保护它们免受消失的相互作用。我们讨论了这一发现对当前旨在避免或延迟由球形芽孢杆菌或苏云金芽孢杆菌柏林毒素控制的害虫产生抗性的策略的后果。

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