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首页> 外文期刊>Journal of Microbiological Methods >TRICORE, a novel bead-based specimen concentration method for the culturing of Mycobacterium tuberculosis
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TRICORE, a novel bead-based specimen concentration method for the culturing of Mycobacterium tuberculosis

机译：TRICORE，一种基于珠子的新型标本浓缩方法，用于结核分枝杆菌的培养

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Centrifugation is a necessary concentrating step for the detection of Mycobacterium tuberculosis in a liquid culture. However, centrifugation is biologically hazardous and presents an obstacle in the development of an automated culture system. A bead-based bacterial concentration method. TRICORE, was recently developed by Genetein Co., Ltd. We compared the efficacy of TRICORE and conventional centrifugation for concentrating M. tuberculosis in clinical sputum specimens by using liquid and solid culture systems. Among 90 pretreated clinical sputum specimens, 51(57.3%) and 55 (61.8%) M. tuberculosis isolates were recovered by the MGIT culture system by using the centrifugation and TRICORE methods, respectively (chi-square test, p =0.5413). The detection time for the centrifugation method was 359.3 +/- 117.0 h, while that for the bead-based concentration method was 377.6 +/- 1623 h (p = 0.5637). However, the number of colonies recovered on solid media were significantly higher with the TRICORE method (p = 0.003). In particular, among the smear-negative specimens, culture positivity of the TRICORE method was 39.6%, while that of the centrifugation method was 15.1%. The TRICORE bead-based concentration method was considered equivalent to centrifugation and enabled efficient collection of paucibacillary specimens in solution. Thus, the new noncentrifugation concentration method could yield more positive culture results
机译：离心是检测液体培养物中结核分枝杆菌的必要浓缩步骤。然而，离心分离对生物有害，并且在自动化培养系统的开发中提出了障碍。基于珠的细菌浓缩方法。 TRICORE是Genetein有限公司最近开发的。我们使用液体和固体培养系统，比较了TRICORE和常规离心浓缩临床痰标本中结核分枝杆菌的功效。在90个经过预处理的临床痰标本中，分别使用离心和TRICORE方法通过MGIT培养系统回收了51.（57.3％）和55（61.8％）的结核分枝杆菌菌株（卡方检验，p = 0.5413）。离心方法的检测时间为359.3 +/- 117.0 h，而基于珠的浓缩方法的检测时间为377.6 +/- 1623 h（p = 0.5637）。但是，使用TRICORE方法在固体培养基上回收的菌落数量明显更高（p = 0.003）。特别地，在涂片阴性样本中，TRICORE方法的培养阳性率为39.6％，而离心方法的培养阳性率为15.1％。基于TRICORE珠的浓缩方法被认为等同于离心分离，能够有效地收集溶液中的脓杆菌标本。因此，新的非离心浓缩方法可以产生更多阳性培养结果

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