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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Formation and structural determinants of multi-stranded guanine-rich DNA complexes.
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Formation and structural determinants of multi-stranded guanine-rich DNA complexes.

机译：多链富含鸟嘌呤的DNA复合物的形成和结构决定因素。

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摘要

We have investigated the complexes formed by oligonucleotides with the general sequence d(T15,Gn), where n = 4-15. Two distinct classes of structures are formed, namely, the four-stranded tetraplex and frayed wires. Frayed wires differ from four-stranded tetraplexes in both strand association stoichiometry and the ability of dimethyl sulfate to methylate the N7 position of guanine. Thus, it appears that these two guanine-rich multistranded assemblies are stabilised by different guanine-guanine interactions. The number of contiguous guanine residues determines which of the complexes is favoured. Based on the stoichiometry of the associated species and the accessibility of the N7 position of guanine to methylation we have found that oligonucleotides with smaller number of contiguous guanines; n = 5-8, form primarily four-stranded tetraplex. Oligonucleotides with larger numbers of contiguous guanines adapt primarily the frayed wire structure. The stability of the complexes formed by this series of oligonucleotides is determined by the number and arrangement of the guanines within the sequences. We propose that the formation of the two types of complex proceed by a parallel reaction pathways that may share common intermediates.
机译：我们已经研究了由具有一般序列d（T15，Gn）的寡核苷酸形成的复合物，其中n = 4-15。形成两种不同类型的结构，即四链四重体和磨损线。磨损线在链缔合化学计量和硫酸二甲酯使鸟嘌呤的N7位置甲基化的能力上均不同于四链的四链体。因此，似乎这两个富含鸟嘌呤的多链组件通过不同的鸟嘌呤-鸟嘌呤相互作用而稳定。连续鸟嘌呤残基的数量决定了哪种复合物是有利的。基于相关物种的化学计量和鸟嘌呤的N7位甲基化的可及性，我们已经发现具有较少连续鸟嘌呤的寡核苷酸;即，具有较低数目的鸟嘌呤的寡核苷酸。 n = 5-8，主要形成四链四链体。具有大量连续鸟嘌呤的寡核苷酸主要适应磨损的金属丝结构。由这一系列寡核苷酸形成的复合物的稳定性取决于序列中鸟嘌呤的数量和排列。我们建议通过可能共享相同中间体的平行反应途径进行两种复合物的形成。

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