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首页> 外文期刊>Journal of Macromolecular Science. Pure and Applied Chemistry >Effect of modifier on enzymatic function of poly[(N-acylimino)ethylene]-modified lipases in organic solvents
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Effect of modifier on enzymatic function of poly[(N-acylimino)ethylene]-modified lipases in organic solvents

机译：改性剂对有机溶剂中聚[（N-酰基酰亚胺）乙烯]修饰的脂肪酶酶功能的影响

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摘要

Candida cylindracea lipase was modi fled by carboxylic-acid-terminated polymers having different molecular weights, i.e., poly[(N-acetylimino)ethylene] (2a), poly[(N-propionylimino)ethylene] (2b) and poly[(N-isobutyrylimino)ethylene] (2c). The effect of N-acyl substituent of 2a-c on the enzymatic activity of modified lipase (4) for hydrolysis of olive oil and p-nitrophenyl propionate in an aqueous solution and for esterification between n-amyl alcohol and caprylic acid in chloroform, benzene, toluene, and isooctane was examined by using 2a-c of a similar MW (5000 similar to 6000). It was found that the active site reactivity of lipase was not as influenced by the chemical modification of lipase in an aqueous medium, and that the rates of esterification were remarkably enhanced, especially in benzene and toluene, by modification with 2b or 2c compared with the native lipase. The effects of the molecular weight of 2a-c and the average number of 2a-c per molecule of lipase on the enzymatic activity were also investigated.
机译：用具有不同分子量的羧酸封端的聚合物，例如聚[（N-乙酰亚氨基）乙烯]（2a），聚[（N-丙酰亚氨基）乙烯]（2b）和聚[（N），修饰念珠菌脂肪酶。 -异丁烯基亚氨基）乙烯]（2c）。 2a-c的N-酰基取代基对修饰的脂肪酶（4）的酶活性的影响，用于水解橄榄油和对硝基苯丙酸酯在水溶液中以及正戊醇和辛酸在氯仿，苯中的酯化甲苯，异辛烷的检测方法是使用2a-c的兆瓦级数（5000类似于6000）。发现在水介质中，脂肪酶的活性位点反应性不受脂肪酶化学修饰的影响，与2b或2c修饰相比，通过2b或2c修饰，酯化速率显着提高，尤其是在苯和甲苯中。天然脂肪酶。还研究了2a-c的分子量和每分子脂肪酶2a-c的平均数对酶活性的影响。

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