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首页> 外文期刊>Journal of Macromolecular Science. Pure and Applied Chemistry >Immobilization of Tyrosinase in Poly(2-thiophen-3-yl-alkyl ester) Derivatives
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Immobilization of Tyrosinase in Poly(2-thiophen-3-yl-alkyl ester) Derivatives

机译：酪氨酸酶在聚（2-噻吩-3-基-烷基酯）衍生物中的固定化

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摘要

In this study, construction of novel biosensors for the determination of phenolic compound was performed via immobilization of tyrosinase during the electrochemical synthesis of conducting block copolymers of 2-thiophen-3-yl-alkyl ester derivatives with 3,4-ethylenedioxythiophene and synthesis of poly(3,4-ethylenedioxythiophene) (PEDOT). The resultant biosensors were characterized in terms of their maximum reaction rates, Michaelis-Menten constants (Km), temperature and pH stabilities. All the copolymer matrices represented higher reaction rates and higher Km values in comparison to both polypyrrole and PEDOT matrices and a relation between the morphology of the matrice and the kinetic parameters was observed. Biosensors maintained their activity even at temperatures as high as 80C and could be used at pHs higher than 8 with high precision. The amount of phenolics in actual samples (red wines) was investigated using electrodes, and results were compared with those found from Folin-Ciocalteau method. Hence, the present study has proven the suitability of these copolymers to be used as polymer matrices for enzyme immobilization.
机译：在这项研究中，通过电化学合成2-噻吩-3-基烷基酯衍生物与3,4-乙撑二氧噻吩的嵌段共聚物和合成聚（3,4-乙撑二氧噻吩）（PEDOT）。所得生物传感器的最大反应速率，Michaelis-Menten常数（Km），温度和pH稳定性对其特性进行了表征。与聚吡咯和PEDOT基质相比，所有共聚物基质均表现出更高的反应速率和更高的Km值，并且观察到了基质的形态与动力学参数之间的关系。生物传感器即使在高达80°C的温度下也能保持其活性，并且可以在pH值高于8的情况下高精度使用。使用电极对实际样品（红酒）中的酚含量进行了调查，并将结果与​​Folin-Ciocalteau方法的结果进行了比较。因此，本研究证明了这些共聚物适合用作酶固定化的聚合物基质。

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