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首页> 外文期刊>Journal of Macromolecular Science. Pure and Applied Chemistry >Experimental approaches to adsorption-desorption dynamism of human serum albumin (HSA) onto crosslinked N,N-diethylaminoethyl (DEAE) dextran microbeads
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Experimental approaches to adsorption-desorption dynamism of human serum albumin (HSA) onto crosslinked N,N-diethylaminoethyl (DEAE) dextran microbeads

机译：人血清白蛋白（HSA）在交联的N，N-二乙基氨基乙基（DEAE）葡聚糖微珠上吸附-解吸动力学的实验方法

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摘要

Crosslinked N,N-Diethylaminoethyl (DEAE) groups containing dextran microbeads have been used in human serum albumin (HSA) adsorption-desorption studies. For the HSA adsorption onto positively charged hydrophilic DEAE dextran microbeads, the adsorption kinetic was slightly decreased by the changing concentration of the protein solution. Adsorption kinetics and equilibrium isotherms for the adsorption of HSA on crosslinked DEAE dextran have been determined experimentally. Modeling of the adsorption processes on DEAE dextran microbeads were realized by applying different adsorption isotherms. Among the several isotherm equations, Langmuir and Freundlich adsorption isotherms were investigated depending on the two temperatures. These were only slightly dependent on the initial concentration of HSA but were considerably affected by the pH of the medium. The HSA adsorption capacity factor and the adsorption equilibrium constant were obtained and mathematical modeling of adsorption, adsorption rate constants and maximum adsorption were determined. Besides the adsorption mechanism, optimum ionic strength and optimum pH also were investigated. Desorption studies and desorption ratio of the system were determined for optimum medium conditions. It was been proved both experimentally and theoretically that human HSA is adsorbed by electrostatic attraction, ion-exchange, hydrophobic interaction and/or hydrogen bonding.
机译：含葡聚糖微珠的交联N，N-二乙基氨基乙基（DEAE）基团已用于人类血清白蛋白（HSA）吸附-解吸研究。对于HSA吸附到带正电荷的亲水性DEAE葡聚糖微珠上，随着蛋白质溶液浓度的变化，吸附动力学略有降低。通过实验确定了HSA在交联DEAE葡聚糖上的吸附动力学和平衡等温线。通过应用不同的吸附等温线，对DEAE葡聚糖微珠的吸附过程进行了建模。在几个等温方程中，根据两个温度研究了Langmuir和Freundlich吸附等温线。这些仅稍微依赖于HSA的初始浓度，但是受培养基pH的影响很大。得到了HSA的吸附容量因子和吸附平衡常数，并确定了吸附，吸附速率常数和最大吸附的数学模型。除了吸附机理外，还研究了最佳离子强度和最佳pH。确定了最佳介质条件下的解吸研究和系统的解吸率。在实验和理论上都证明了人HSA通过静电吸引，离子交换，疏水相互作用和/或氢键结合而被吸附。

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